Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production
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2019
Authors
Buntić, AnetaMilić, Marija
Stajković-Srbinović, Olivera S.
Rasulić, Nataša V.
Delić, Dušica I.
Mihajlovski, Katarina
Article (Published version)
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This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl ce...llulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.
Keywords:
Avicelase / Carboxymethyl cellulase / Lignocellulose / Response surface method / RhizobiumSource:
International Journal of Environmental Science and Technology, 2019, 16, 10, 5881-5890Publisher:
- Springer, New York
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-MESTD-Technological Development (TD or TR)-31035)
- Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection (RS-MESTD-Technological Development (TD or TR)-37006)
DOI: 10.1007/s13762-019-02230-9
ISSN: 1735-1472
WoS: 000486155600043
Scopus: 2-s2.0-85060716746
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Buntić, Aneta AU - Milić, Marija AU - Stajković-Srbinović, Olivera S. AU - Rasulić, Nataša V. AU - Delić, Dušica I. AU - Mihajlovski, Katarina PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4075 AB - This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste. PB - Springer, New York T2 - International Journal of Environmental Science and Technology T1 - Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production EP - 5890 IS - 10 SP - 5881 VL - 16 DO - 10.1007/s13762-019-02230-9 ER -
@article{ author = "Buntić, Aneta and Milić, Marija and Stajković-Srbinović, Olivera S. and Rasulić, Nataša V. and Delić, Dušica I. and Mihajlovski, Katarina", year = "2019", abstract = "This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.", publisher = "Springer, New York", journal = "International Journal of Environmental Science and Technology", title = "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production", pages = "5890-5881", number = "10", volume = "16", doi = "10.1007/s13762-019-02230-9" }
Buntić, A., Milić, M., Stajković-Srbinović, O. S., Rasulić, N. V., Delić, D. I.,& Mihajlovski, K.. (2019). Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology Springer, New York., 16(10), 5881-5890. https://doi.org/10.1007/s13762-019-02230-9
Buntić A, Milić M, Stajković-Srbinović OS, Rasulić NV, Delić DI, Mihajlovski K. Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology. 2019;16(10):5881-5890. doi:10.1007/s13762-019-02230-9 .
Buntić, Aneta, Milić, Marija, Stajković-Srbinović, Olivera S., Rasulić, Nataša V., Delić, Dušica I., Mihajlovski, Katarina, "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production" in International Journal of Environmental Science and Technology, 16, no. 10 (2019):5881-5890, https://doi.org/10.1007/s13762-019-02230-9 . .