Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
Samo za registrovane korisnike
2019
Autori
Lazarević, J. J.Ralević, U.
Kukolj, Tamara
Bugarski, Diana
Lazarević, N.
Bugarski, Branko
Popović, Z., V
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different... chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
Ključne reči:
Raman spectroscopy / Stem cells / Chemical fixationIzvor:
Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2019, 216, 173-178Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Nanostrukturni multifunkcionalni materijali i nanokompoziti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45018)
- Regenerativni i modulatorni potencijal adultnih matičnih ćelija (RS-MESTD-Basic Research (BR or ON)-175062)
- Fizika uređenih nanostruktura i novih materijala u fotonici (RS-MESTD-Basic Research (BR or ON)-171005)
DOI: 10.1016/j.saa.2019.03.012
ISSN: 1386-1425
PubMed: 30897378
WoS: 000471317300021
Scopus: 2-s2.0-85062988764
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Lazarević, J. J. AU - Ralević, U. AU - Kukolj, Tamara AU - Bugarski, Diana AU - Lazarević, N. AU - Bugarski, Branko AU - Popović, Z., V PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4213 AB - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy T1 - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy EP - 178 SP - 173 VL - 216 DO - 10.1016/j.saa.2019.03.012 ER -
@article{ author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V", year = "2019", abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy", title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy", pages = "178-173", volume = "216", doi = "10.1016/j.saa.2019.03.012" }
Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178. https://doi.org/10.1016/j.saa.2019.03.012
Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2019;216:173-178. doi:10.1016/j.saa.2019.03.012 .
Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 216 (2019):173-178, https://doi.org/10.1016/j.saa.2019.03.012 . .