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Imobilizacija Aspergillus Niger celulaze na Lifetech TM nosače i primena u reakciji hidrolize lignocelulozne frakcije suncokretove sačme

dc.creatorĆorović, Marija
dc.creatorSimović, Milica
dc.creatorMilivojević, Ana
dc.creatorBanjanac, Katarina
dc.creatorKatić, Katarina D.
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T14:10:03Z
dc.date.available2021-03-10T14:10:03Z
dc.date.issued2019
dc.identifier.issn2217-5369
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4299
dc.description.abstractCellulases are enzymes which catalyse cellulose hydrolysis and are widely used in various industry branches. Lately, their application in treatment of different agroindustrial waste materials which could serve for fuel production is being extensively explored. In order to increase their stability and cost-effectiveness of their usage, application of their immobilized forms are preferred over free enzymes. Hereby, we tested eight different LifetechTM immobilization supports differing in polarity, porosity and functional groups as carriers for Asspergillus Niger cellulase immobilization. Most promising carrier was methacrylate based, with primary amino groups, C6 "space arm" and pores with diameter of 60-120 nm - LifetechTM ECR8409F. For this support, most important immobilization parameters were investigated and after 3 h at pH 6 with initial protein concentration of 23.3 mg/g support immobilized cellulase with 406 IU/g (with carboxymethyl cellulose as a substrate) was obtained. This preparation was successfully applied in the hydrolysis of lignocellulosic fraction of sunflower seed meal, which is widely available byproduct of sunflower seed meal fractionation for protein-rich fractions production. Initial reaction rates and yields of reducing sugars were unchanged comparing to free enzyme, indicating that there were no significant diffusion limitations for substrate to approach active sites of A. Niger cellulase molecules immobilized onto LifetechTM ECR8409F support.en
dc.description.abstractCelulaze su enzimi koji katalizuju hidrolizu celuloze i u širokoj su primeni u brojnim granama industrije. U poslednje vreme, mogućnost njihove primene u tretiranju različitih opadnih agroindustrijskih sirovina u cilju dobijanja biogoriva intenzivno se istražuje. Da bi se povećale stabilnost i ekonomičnost njihove primene, potrebno je umesto slobodnog enzima koristiti imobilisane forme. U ovom istraživanju osam LifetechTM nosača različitih polarnosti, poroznosti i funkcionalnih grupa. testirano je za imobilizaciju celulaze producenta Aspergillus Niger. Najpogodniji nosač bio je na bazi metakrilata, sa primarnim amino grupama, C6 "dugom nožicom" i porama prečnika 60-120 nm - LifetechTM ECR8409F. Za ovaj nosač određeni su najznačajniji uslovi imobilizacije i nakon 3 sata na pH 6, pri početnoj koncentraciji proteina od 23,3 mg/g nosača dobijena je imobilisana celulaza aktivnosti 406 IU/g (sa karboksi metil celulozom kao supstratom). Ovaj preparat uspešno je primenjen u reakciji hidrolize lignocelulozne frakcije suncokretove sačme, koja prestavlja nusproizvod frakcionisanja suncokretove sačme pri dobijanju frakcija bogatih proteinima. Početne brzine reakcije i prinosi redukujućih šećera bili su nepromenjeni u odnosu na slobodan enzim, ukazujući da nije bilo značajnog uticaja difuzionih limitacija pri prilasku supstrata aktivnim centrima molekula A. Niger celulaze imobilisane na LifetechTM ECR8409F nosač.sr
dc.publisherInstitute for Food Technology, Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceFood and Feed Research
dc.subjectb-glucanaseen
dc.subjectimmobilizationen
dc.subjectsunflower seed mealen
dc.subjectlignocelluloseen
dc.subjectsaccharificationen
dc.subjectb-glukanazasr
dc.subjectimobilizacijasr
dc.subjectsuncokretova sačmasr
dc.subjectlignocelulozasr
dc.subjectsaharifikacijasr
dc.titleImmobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fractionen
dc.titleImobilizacija Aspergillus Niger celulaze na Lifetech TM nosače i primena u reakciji hidrolize lignocelulozne frakcije suncokretove sačmesr
dc.typearticle
dc.rights.licenseBY
dc.citation.epage169
dc.citation.issue2
dc.citation.other46(2): 161-169
dc.citation.rankM24
dc.citation.spage161
dc.citation.volume46
dc.identifier.doi10.5937/FFR1902161C
dc.identifier.rcubconv_1794
dc.type.versionpublishedVersion


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