The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment
Authors
Buntić, AnetaStajković-Srbinović, Olivera S.
Delić, Dušica I.
Dimitrijević-Branković, Suzana
Milić, Marija
Article (Published version)
Metadata
Show full item recordAbstract
Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine incr...eased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1.
Keywords:
lignocellulosic waste / microwave-assisted extraction / solid-state fermentation / CMCase and avicelase activitySource:
Journal of the Serbian Chemical Society, 2019, 84, 2, 129-140Publisher:
- Srpsko hemijsko društvo, Beograd
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-MESTD-Technological Development (TD or TR)-31035)
- Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection (RS-MESTD-Technological Development (TD or TR)-37006)
DOI: 10.2298/JSC180802114B
ISSN: 0352-5139
WoS: 000460644600002
Scopus: 2-s2.0-85065423643
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Buntić, Aneta AU - Stajković-Srbinović, Olivera S. AU - Delić, Dušica I. AU - Dimitrijević-Branković, Suzana AU - Milić, Marija PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4311 AB - Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment EP - 140 IS - 2 SP - 129 VL - 84 DO - 10.2298/JSC180802114B ER -
@article{ author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Delić, Dušica I. and Dimitrijević-Branković, Suzana and Milić, Marija", year = "2019", abstract = "Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment", pages = "140-129", number = "2", volume = "84", doi = "10.2298/JSC180802114B" }
Buntić, A., Stajković-Srbinović, O. S., Delić, D. I., Dimitrijević-Branković, S.,& Milić, M.. (2019). The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 84(2), 129-140. https://doi.org/10.2298/JSC180802114B
Buntić A, Stajković-Srbinović OS, Delić DI, Dimitrijević-Branković S, Milić M. The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment. in Journal of the Serbian Chemical Society. 2019;84(2):129-140. doi:10.2298/JSC180802114B .
Buntić, Aneta, Stajković-Srbinović, Olivera S., Delić, Dušica I., Dimitrijević-Branković, Suzana, Milić, Marija, "The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment" in Journal of the Serbian Chemical Society, 84, no. 2 (2019):129-140, https://doi.org/10.2298/JSC180802114B . .