Validation of a novel perfusion bioreactor system in cancer research
Аутори
Stojkovska, JasminaZvicer, Jovana
Milivojević, Milena
Petrović, Isidora
Stevanović, Milena
Obradović, Bojana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in s...hort term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
Кључне речи:
3D culture / biomimetic bioreactor / tumor model / alginate microfibersИзвор:
Hemijska industrija, 2020, 74, 3, 187-196Издавач:
- Savez hemijskih inženjera, Beograd
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
DOI: 10.2298/HEMIND200329015S
ISSN: 0367-598X
WoS: 000577922500003
Scopus: 2-s2.0-85087435489
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Stojkovska, Jasmina AU - Zvicer, Jovana AU - Milivojević, Milena AU - Petrović, Isidora AU - Stevanović, Milena AU - Obradović, Bojana PY - 2020 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4373 AB - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment. PB - Savez hemijskih inženjera, Beograd T2 - Hemijska industrija T1 - Validation of a novel perfusion bioreactor system in cancer research EP - 196 IS - 3 SP - 187 VL - 74 DO - 10.2298/HEMIND200329015S ER -
@article{ author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana", year = "2020", abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Hemijska industrija", title = "Validation of a novel perfusion bioreactor system in cancer research", pages = "196-187", number = "3", volume = "74", doi = "10.2298/HEMIND200329015S" }
Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija Savez hemijskih inženjera, Beograd., 74(3), 187-196. https://doi.org/10.2298/HEMIND200329015S
Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija. 2020;74(3):187-196. doi:10.2298/HEMIND200329015S .
Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska industrija, 74, no. 3 (2020):187-196, https://doi.org/10.2298/HEMIND200329015S . .