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dc.creatorBebić, Jelena
dc.creatorBanjanac, Katarina
dc.creatorĆorović, Marija
dc.creatorMilivojević, Ana
dc.creatorSimović, Milica
dc.creatorMarinković, Aleksandar
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T14:15:27Z
dc.date.available2021-03-10T14:15:27Z
dc.date.issued2020
dc.identifier.issn1004-9541
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4384
dc.description.abstractThis work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.en
dc.publisherChemical Industry Press Co Ltd, Beijing
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45019/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS//
dc.rightsrestrictedAccess
dc.sourceChinese Journal of Chemical Engineering
dc.subjectSilica nanoparticlesen
dc.subjectLaccaseen
dc.subjectEnzyme immobilizationen
dc.subjectLindaneen
dc.subjectBioremediationen
dc.titleImmobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1144
dc.citation.issue4
dc.citation.other28(4): 1136-1144
dc.citation.rankM22~
dc.citation.spage1136
dc.citation.volume28
dc.identifier.doi10.1016/j.cjche.2019.12.025
dc.identifier.rcubconv_6181
dc.identifier.scopus2-s2.0-85081279850
dc.identifier.wos000555483300022
dc.type.versionpublishedVersion


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