TechnoRep - Faculty of Technology and Metallurgy Repository
University of Belgrade - Faculty of Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Bioreactor studies of native and tissue engineered cartilage

No Thumbnail
Authors
Vunjak-Novaković, Gordana
Obradović, Bojana
Martin, Ivan
Freed, LE
Conference object (Published version)
Metadata
Show full item record
Abstract
Functional tissue engineering of cartilage involves the use of bioreactors designed to provide a controlled in vitro environment that embodies some of the biochemical and physical signals known to regulate chondrogenesis. Hydrodynamic conditions can affect in vitro tissue formation in at least two ways: by direct effects of hydrodynamic forces on cell morphology and function, and by indirect flow-induced changes in mass transfer of nutrients and metabolites. In the present work, we discuss the effects of three different in vitro environments: static flasks (tissues fixed in place, static medium), mixed flasks (tissues fixed in place, unidirectional turbulent flow) and rotating bioreactors (tissues dynamically suspended in laminar flow) on engineered cartilage constructs and native cartilage explants. As compared to static and mixed flasks, dynamic laminar flow in rotating bioreactors resulted in the most rapid tissue growth and the highest final fractions of glycosaminoglycans and tota...l collagen in both tissues. Mechanical properties (equilibrium modulus, dynamic stiffness, hydraulic permeability) of engineered constructs and explanted cartilage correlated with the wet weight fractions of glycosaminoglycans and collagen. Current research needs in the area of cartilage tissue engineering include the utilization of additional physiologically relevant regulatory signals, and the development of predictive mathematical models that enable optimization of the conditions and duration of tissue culture.

Source:
Biorheology, 2002, 39, 1-2, 259-268
Publisher:
  • IOS Press, Amsterdam

ISSN: 0006-355X

PubMed: 12082288

WoS: 000175769100028

[ Google Scholar ]
78
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/445
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - CONF
AU  - Vunjak-Novaković, Gordana
AU  - Obradović, Bojana
AU  - Martin, Ivan
AU  - Freed, LE
PY  - 2002
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/445
AB  - Functional tissue engineering of cartilage involves the use of bioreactors designed to provide a controlled in vitro environment that embodies some of the biochemical and physical signals known to regulate chondrogenesis. Hydrodynamic conditions can affect in vitro tissue formation in at least two ways: by direct effects of hydrodynamic forces on cell morphology and function, and by indirect flow-induced changes in mass transfer of nutrients and metabolites. In the present work, we discuss the effects of three different in vitro environments: static flasks (tissues fixed in place, static medium), mixed flasks (tissues fixed in place, unidirectional turbulent flow) and rotating bioreactors (tissues dynamically suspended in laminar flow) on engineered cartilage constructs and native cartilage explants. As compared to static and mixed flasks, dynamic laminar flow in rotating bioreactors resulted in the most rapid tissue growth and the highest final fractions of glycosaminoglycans and total collagen in both tissues. Mechanical properties (equilibrium modulus, dynamic stiffness, hydraulic permeability) of engineered constructs and explanted cartilage correlated with the wet weight fractions of glycosaminoglycans and collagen. Current research needs in the area of cartilage tissue engineering include the utilization of additional physiologically relevant regulatory signals, and the development of predictive mathematical models that enable optimization of the conditions and duration of tissue culture.
PB  - IOS Press, Amsterdam
C3  - Biorheology
T1  - Bioreactor studies of native and tissue engineered cartilage
EP  - 268
IS  - 1-2
SP  - 259
VL  - 39
UR  - conv_2208
ER  - 
@conference{
author = "Vunjak-Novaković, Gordana and Obradović, Bojana and Martin, Ivan and Freed, LE",
year = "2002",
abstract = "Functional tissue engineering of cartilage involves the use of bioreactors designed to provide a controlled in vitro environment that embodies some of the biochemical and physical signals known to regulate chondrogenesis. Hydrodynamic conditions can affect in vitro tissue formation in at least two ways: by direct effects of hydrodynamic forces on cell morphology and function, and by indirect flow-induced changes in mass transfer of nutrients and metabolites. In the present work, we discuss the effects of three different in vitro environments: static flasks (tissues fixed in place, static medium), mixed flasks (tissues fixed in place, unidirectional turbulent flow) and rotating bioreactors (tissues dynamically suspended in laminar flow) on engineered cartilage constructs and native cartilage explants. As compared to static and mixed flasks, dynamic laminar flow in rotating bioreactors resulted in the most rapid tissue growth and the highest final fractions of glycosaminoglycans and total collagen in both tissues. Mechanical properties (equilibrium modulus, dynamic stiffness, hydraulic permeability) of engineered constructs and explanted cartilage correlated with the wet weight fractions of glycosaminoglycans and collagen. Current research needs in the area of cartilage tissue engineering include the utilization of additional physiologically relevant regulatory signals, and the development of predictive mathematical models that enable optimization of the conditions and duration of tissue culture.",
publisher = "IOS Press, Amsterdam",
journal = "Biorheology",
title = "Bioreactor studies of native and tissue engineered cartilage",
pages = "268-259",
number = "1-2",
volume = "39",
url = "conv_2208"
}
Vunjak-Novaković, G., Obradović, B., Martin, I.,& Freed, L.. (2002). Bioreactor studies of native and tissue engineered cartilage. in Biorheology
IOS Press, Amsterdam., 39(1-2), 259-268.
conv_2208
Vunjak-Novaković G, Obradović B, Martin I, Freed L. Bioreactor studies of native and tissue engineered cartilage. in Biorheology. 2002;39(1-2):259-268.
conv_2208 .
Vunjak-Novaković, Gordana, Obradović, Bojana, Martin, Ivan, Freed, LE, "Bioreactor studies of native and tissue engineered cartilage" in Biorheology, 39, no. 1-2 (2002):259-268,
conv_2208 .

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB