Considerations for the design and conduct of human gut microbiota intervention studies relating to foods
Authors
Swann, J. R.Rajilić-Stojanović, Mirjana
Salonen, Anne
Sakwinska, O.
Gill, C.
Meynier, A.
Fanca-Berthon, P.
Schelkle, B.
Segata, N.
Shortt, C.
Tuohy, K.
Hasselwander, O.
Article (Published version)
Metadata
Show full item recordAbstract
With the growing appreciation for the influence of the intestinal microbiota on human health, there is increasing motivation to design and refine interventions to promote favorable shifts in the microbiota and their interactions with the host. Technological advances have improved our understanding and ability to measure this indigenous population and the impact of such interventions. However, the rapid growth and evolution of the field, as well as the diversity of methods used, parameters measured and populations studied, make it difficult to interpret the significance of the findings and translate their outcomes to the wider population. This can prevent comparisons across studies and hinder the drawing of appropriate conclusions. This review outlines considerations to facilitate the design, implementation and interpretation of human gut microbiota intervention studies relating to foods based upon our current understanding of the intestinal microbiota, its functionality and interaction...s with the human host. This includes parameters associated with study design, eligibility criteria, statistical considerations, characterization of products and the measurement of compliance. Methodologies and markers to assess compositional and functional changes in the microbiota, following interventions are discussed in addition to approaches to assess changes in microbiota-host interactions and host responses. Last, EU legislative aspects in relation to foods and health claims are presented. While it is appreciated that the field of gastrointestinal microbiology is rapidly evolving, such guidance will assist in the design and interpretation of human gut microbiota interventional studies relating to foods.
Keywords:
Microbiota / Microbiome / Gut bacteria / Intestinal microbes / Human studies / Foods / Prebiotics / ProbioticsSource:
European Journal of Nutrition, 2020, 59, 8, 3347-3368Publisher:
- Springer Heidelberg, Heidelberg
DOI: 10.1007/s00394-020-02232-1
ISSN: 1436-6207
PubMed: 32246263
WoS: 000523112300003
Scopus: 2-s2.0-85083279259
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Swann, J. R. AU - Rajilić-Stojanović, Mirjana AU - Salonen, Anne AU - Sakwinska, O. AU - Gill, C. AU - Meynier, A. AU - Fanca-Berthon, P. AU - Schelkle, B. AU - Segata, N. AU - Shortt, C. AU - Tuohy, K. AU - Hasselwander, O. PY - 2020 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4473 AB - With the growing appreciation for the influence of the intestinal microbiota on human health, there is increasing motivation to design and refine interventions to promote favorable shifts in the microbiota and their interactions with the host. Technological advances have improved our understanding and ability to measure this indigenous population and the impact of such interventions. However, the rapid growth and evolution of the field, as well as the diversity of methods used, parameters measured and populations studied, make it difficult to interpret the significance of the findings and translate their outcomes to the wider population. This can prevent comparisons across studies and hinder the drawing of appropriate conclusions. This review outlines considerations to facilitate the design, implementation and interpretation of human gut microbiota intervention studies relating to foods based upon our current understanding of the intestinal microbiota, its functionality and interactions with the human host. This includes parameters associated with study design, eligibility criteria, statistical considerations, characterization of products and the measurement of compliance. Methodologies and markers to assess compositional and functional changes in the microbiota, following interventions are discussed in addition to approaches to assess changes in microbiota-host interactions and host responses. Last, EU legislative aspects in relation to foods and health claims are presented. While it is appreciated that the field of gastrointestinal microbiology is rapidly evolving, such guidance will assist in the design and interpretation of human gut microbiota interventional studies relating to foods. PB - Springer Heidelberg, Heidelberg T2 - European Journal of Nutrition T1 - Considerations for the design and conduct of human gut microbiota intervention studies relating to foods EP - 3368 IS - 8 SP - 3347 VL - 59 DO - 10.1007/s00394-020-02232-1 ER -
@article{ author = "Swann, J. R. and Rajilić-Stojanović, Mirjana and Salonen, Anne and Sakwinska, O. and Gill, C. and Meynier, A. and Fanca-Berthon, P. and Schelkle, B. and Segata, N. and Shortt, C. and Tuohy, K. and Hasselwander, O.", year = "2020", abstract = "With the growing appreciation for the influence of the intestinal microbiota on human health, there is increasing motivation to design and refine interventions to promote favorable shifts in the microbiota and their interactions with the host. Technological advances have improved our understanding and ability to measure this indigenous population and the impact of such interventions. However, the rapid growth and evolution of the field, as well as the diversity of methods used, parameters measured and populations studied, make it difficult to interpret the significance of the findings and translate their outcomes to the wider population. This can prevent comparisons across studies and hinder the drawing of appropriate conclusions. This review outlines considerations to facilitate the design, implementation and interpretation of human gut microbiota intervention studies relating to foods based upon our current understanding of the intestinal microbiota, its functionality and interactions with the human host. This includes parameters associated with study design, eligibility criteria, statistical considerations, characterization of products and the measurement of compliance. Methodologies and markers to assess compositional and functional changes in the microbiota, following interventions are discussed in addition to approaches to assess changes in microbiota-host interactions and host responses. Last, EU legislative aspects in relation to foods and health claims are presented. While it is appreciated that the field of gastrointestinal microbiology is rapidly evolving, such guidance will assist in the design and interpretation of human gut microbiota interventional studies relating to foods.", publisher = "Springer Heidelberg, Heidelberg", journal = "European Journal of Nutrition", title = "Considerations for the design and conduct of human gut microbiota intervention studies relating to foods", pages = "3368-3347", number = "8", volume = "59", doi = "10.1007/s00394-020-02232-1" }
Swann, J. R., Rajilić-Stojanović, M., Salonen, A., Sakwinska, O., Gill, C., Meynier, A., Fanca-Berthon, P., Schelkle, B., Segata, N., Shortt, C., Tuohy, K.,& Hasselwander, O.. (2020). Considerations for the design and conduct of human gut microbiota intervention studies relating to foods. in European Journal of Nutrition Springer Heidelberg, Heidelberg., 59(8), 3347-3368. https://doi.org/10.1007/s00394-020-02232-1
Swann JR, Rajilić-Stojanović M, Salonen A, Sakwinska O, Gill C, Meynier A, Fanca-Berthon P, Schelkle B, Segata N, Shortt C, Tuohy K, Hasselwander O. Considerations for the design and conduct of human gut microbiota intervention studies relating to foods. in European Journal of Nutrition. 2020;59(8):3347-3368. doi:10.1007/s00394-020-02232-1 .
Swann, J. R., Rajilić-Stojanović, Mirjana, Salonen, Anne, Sakwinska, O., Gill, C., Meynier, A., Fanca-Berthon, P., Schelkle, B., Segata, N., Shortt, C., Tuohy, K., Hasselwander, O., "Considerations for the design and conduct of human gut microbiota intervention studies relating to foods" in European Journal of Nutrition, 59, no. 8 (2020):3347-3368, https://doi.org/10.1007/s00394-020-02232-1 . .