Приказ основних података о документу

dc.creatorStančić, Ana
dc.creatorDrvenica, Ivana
dc.creatorObradović, Hristina
dc.creatorBugarski, Branko
dc.creatorIlić, Vesna Lj.
dc.creatorBugarski, Diana
dc.date.accessioned2021-03-10T14:21:45Z
dc.date.available2021-03-10T14:21:45Z
dc.date.issued2020
dc.identifier.issn0141-8130
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4483
dc.description.abstractWe have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.en
dc.publisherElsevier, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/146010/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectExtracellular hemoglobinen
dc.subjectBovineen
dc.subjectMesenchymal stromal cellsen
dc.subjectProliferationen
dc.subjectMigrationen
dc.subjectDifferentiation capacityen
dc.titleNative bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitroen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage920
dc.citation.other144: 909-920
dc.citation.rankM21
dc.citation.spage909
dc.citation.volume144
dc.identifier.doi10.1016/j.ijbiomac.2019.09.167
dc.identifier.pmid31669467
dc.identifier.scopus2-s2.0-85075878005
dc.identifier.wos000515200700094
dc.type.versionpublishedVersion


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Приказ основних података о документу