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dc.creatorStevanović, Milena
dc.creatorĐošić, Marija
dc.creatorJanković, Ana
dc.creatorNešović, Katarina
dc.creatorKojić, Vesna
dc.creatorStojanović, Jovica
dc.creatorGrujić, Svetlana
dc.creatorMatić-Bujagić, Ivana
dc.creatorRhee, Kyong Yop
dc.creatorMišković-Stanković, Vesna
dc.date.accessioned2021-03-10T14:22:45Z
dc.date.available2021-03-10T14:22:45Z
dc.date.issued2020
dc.identifier.issn2470-1343
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4498
dc.description.abstractThe electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability gt 85%), providing a promising basis for further medical application trials.en
dc.publisherAmer Chemical Soc, Washington
dc.relationBasic Science Research Program of the Ministry of Education, Science and Technology of Korea [2018R1A2B5A02023190]
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceACS Omega
dc.titleAssessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrateen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage15445
dc.citation.issue25
dc.citation.other5(25): 15433-15445
dc.citation.rankM22
dc.citation.spage15433
dc.citation.volume5
dc.identifier.doi10.1021/acsomega.0c01583
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/2073/4495.pdf
dc.identifier.pmid32637818
dc.identifier.scopus2-s2.0-85087796063
dc.identifier.wos000546100300061
dc.type.versionpublishedVersion


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