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Amino-modified kraft lignin microspheres as a support for enzyme immobilization
dc.creator | Bebić, Jelena | |
dc.creator | Banjanac, Katarina | |
dc.creator | Rusmirović, Jelena | |
dc.creator | Ćorović, Marija | |
dc.creator | Milivojević, Ana | |
dc.creator | Simović, Milica | |
dc.creator | Marinković, Aleksandar | |
dc.creator | Bezbradica, Dejan | |
dc.date.accessioned | 2021-03-10T14:23:31Z | |
dc.date.available | 2021-03-10T14:23:31Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 2046-2069 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4510 | |
dc.description.abstract | In this research, it has been demonstrated that amino-modified microspheres (A-LMS) based on bio-waste derived material, such as kraft lignin, have good prospects in usage as a support for enzyme immobilization, since active biocatalyst systems were prepared by immobilizing beta-galactosidase fromA. oryzaeand laccase fromM. thermophilaexpressed inA. oryzae(Novozym (R) 51003) onto A-LMS. Two types of A-LMS were investigated, with different emulsifier concentrations (5 wt% and 10 wt%), and microspheres produced using 5 wt% of emulsifier (A-LMS_5) showed adequate pore shape, size and distribution for enzyme attachment. The type of interactions formed between enzymes (beta-galactosidase and laccase) and A-LMS_5 microspheres demonstrated that beta-galactosidase is predominantly attachedviaelectrostatic interactions while attachment of laccase is equally governed by electrostatic and hydrophobic interactions. Furthermore, the A-LMS_5-beta-galactosidase exhibited specificity towards recognized prebiotics (galacto-oligosaccharides (GOS)) synthesis with 1.5-times higher GOS production than glucose production, while for environmental pollutant lindane degradation, the immobilized laccase preparation exhibited high activity with a minimum remaining lindane concentration of 22.4% after 6 days. Thus, this novel enzyme immobilization support A-LMS_5 has potential for use in green biotechnologies. | en |
dc.publisher | Royal Society of Chemistry | |
dc.relation | COST (European Cooperation in Science and Technology)European Cooperation in Science and Technology (COST) [CA17128] | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.source | RSC Advances | |
dc.title | Amino-modified kraft lignin microspheres as a support for enzyme immobilization | en |
dc.type | article | |
dc.rights.license | BY-NC | |
dc.citation.epage | 21508 | |
dc.citation.issue | 36 | |
dc.citation.other | 10(36): 21495-21508 | |
dc.citation.rank | M22 | |
dc.citation.spage | 21495 | |
dc.citation.volume | 10 | |
dc.identifier.doi | 10.1039/d0ra03439h | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/10214/d0ra03439h.pdf | |
dc.identifier.scopus | 2-s2.0-85086520859 | |
dc.identifier.wos | 000542722900049 | |
dc.type.version | publishedVersion |