Production and characterization of nanocomposite hydrogels based on alginate and silver nanoparticles for biomedical applications

Abstract

Development of novel nanocomposite biomaterials for biomedical applications requires comprehensive evaluation and characterization of biomaterials such as determination of the chemical composition, physical properties, biocompatibility and functionality. In addition, successful development of biomaterials for potential biomedical applications requires all inclusive association of all the results for reliable prediction of the behavior and functionality of biomaterials in the desired application. The aim of this doctoral thesis was to investigate possibilities for production of antimicrobial hydrogels in different forms from alginate colloid solutions with electrochemically synthesized silver nanoparticles, as well as comprehensive evaluation of the obtained nanocomposits under in vitro and in vivo conditions. In vitro studies included assessment of cytotoxicity, biomechanical properties, antimicrobial activity and silver release, while in vivo studies comprised functionality evaluation of the nanocomposites for potential biomedical applications in wound tretman...

Razvoj novih nanokompozitnih biomaterijala za biomedicinsku primenu zahteva različita ispitivanja i karakterisanje biomaterijala kako sa stanovišta hemijskog sastava i fizičkih svojstava, tako i sa stanovišta biokompatibilnosti i funkcionalnosti. Uz to, da bi se razvio optimizovan biomaterijal potrebno je rezultate svih ispitivanja povezati i objasniti radi mogućnosti pouzdanog predviđanja ponašanja i funkcije biomaterijala u željenoj bimedicinskoj primeni. Cilj istraživanja u ovoj doktorskoj disertaciji je bio ispitivanje mogućnosti dobijanja antimikrobnih hidrogelova u različitim oblicima iz koloidnih rastvora alginata sa elektrohemijski sintetisanim nanočesticama srebra i sveobuhvatna karakterizacija proizvedenih nanokompozita u in vitro i in vivo uslovima. In vitro karakterizacija je obuhvatila ispitivanja citotoksičnosti, biomehaničkih karakteristika, antimikrobne aktivnosti i otpuštanja srebra, dok je u in vivo studijama ispitana funkcionalnost nanokompozita za potencijalnu biomedicinsku primenu u tretmanu rana...