Safe-by-design gelatin-modified zinc oxide nanoparticles
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2021
Authors
Janicijevic, ZeljkoStankovic, Ana
Zegura, Bojana
Veljović, Đorđe
Djekic, Ljiljana
Krajisnik, Danina
Filipic, Metka
Stevanovic, Magdalena M.
Article (Published version)
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We report an innovative low-cost wet precipitation synthesis method for gelatin-modified zinc oxide nanoparticles (GM ZnO NPs) at the interface between the gelatin hydrogel and aqueous electrolyte. Diffusion of ammonia through the hydrogel matrices with different gelatin contents induced precipitation of the product in contact with the surface of the aqueous solution of zinc ions. The obtained precipitate was subjected to thermal treatment to partially decompose the adsorbed gelatin in the NP structure. Physicochemical properties of obtained GM ZnO NPs were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), differential thermal analysis (DTA), thermogravimetry (TG), photon correlation spectroscopy (PCS), zeta potential measurements, and inductively coupled plasma-mass spectrometry (ICP-MS). The estimated mean crystallite size of GM ZnO NP powders was in the range from 5.8 to 12.1 nm. The synthesized NPs e...xhibited nanosheet morphology and arranged into flake-like aggregates. The toxic potential was investigated in vitro in human hepatocellular carcinoma cell line HepG2. The thiazolyl blue tetrazolium bromide (MTS) assay was used to assess cell viability, 2 ',7 '-dichlor-fluorescein-diacetate (DCFH-DA) assay to examine the formation of intracellular reactive oxygen species (ROS), and comet assay to evaluate the genotoxic response. GM ZnO NPs slightly reduced HepG2 cell viability, did not induce ROS formation, and showed low genotoxic potential at very high doses (100 mu g mL(-1)). ZnO NPs fabricated and modified using the proposed methodology deserve further study as potential candidates for antibacterial agents or dietary supplements with low overall toxicity.
Keywords:
ZnO / Nanoparticles / Precipitation / Gelatin / Hydrogel / Toxicity / Health effectsSource:
Journal of Nanoparticle Research, 2021, 23, 9Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-MESTD-inst-2020-200175)
DOI: 10.1007/s11051-021-05312-3
ISSN: 1388-0764
WoS: 000690951900002
Scopus: 2-s2.0-85113624081
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Janicijevic, Zeljko AU - Stankovic, Ana AU - Zegura, Bojana AU - Veljović, Đorđe AU - Djekic, Ljiljana AU - Krajisnik, Danina AU - Filipic, Metka AU - Stevanovic, Magdalena M. PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4766 AB - We report an innovative low-cost wet precipitation synthesis method for gelatin-modified zinc oxide nanoparticles (GM ZnO NPs) at the interface between the gelatin hydrogel and aqueous electrolyte. Diffusion of ammonia through the hydrogel matrices with different gelatin contents induced precipitation of the product in contact with the surface of the aqueous solution of zinc ions. The obtained precipitate was subjected to thermal treatment to partially decompose the adsorbed gelatin in the NP structure. Physicochemical properties of obtained GM ZnO NPs were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), differential thermal analysis (DTA), thermogravimetry (TG), photon correlation spectroscopy (PCS), zeta potential measurements, and inductively coupled plasma-mass spectrometry (ICP-MS). The estimated mean crystallite size of GM ZnO NP powders was in the range from 5.8 to 12.1 nm. The synthesized NPs exhibited nanosheet morphology and arranged into flake-like aggregates. The toxic potential was investigated in vitro in human hepatocellular carcinoma cell line HepG2. The thiazolyl blue tetrazolium bromide (MTS) assay was used to assess cell viability, 2 ',7 '-dichlor-fluorescein-diacetate (DCFH-DA) assay to examine the formation of intracellular reactive oxygen species (ROS), and comet assay to evaluate the genotoxic response. GM ZnO NPs slightly reduced HepG2 cell viability, did not induce ROS formation, and showed low genotoxic potential at very high doses (100 mu g mL(-1)). ZnO NPs fabricated and modified using the proposed methodology deserve further study as potential candidates for antibacterial agents or dietary supplements with low overall toxicity. T2 - Journal of Nanoparticle Research T1 - Safe-by-design gelatin-modified zinc oxide nanoparticles IS - 9 VL - 23 DO - 10.1007/s11051-021-05312-3 ER -
@article{ author = "Janicijevic, Zeljko and Stankovic, Ana and Zegura, Bojana and Veljović, Đorđe and Djekic, Ljiljana and Krajisnik, Danina and Filipic, Metka and Stevanovic, Magdalena M.", year = "2021", abstract = "We report an innovative low-cost wet precipitation synthesis method for gelatin-modified zinc oxide nanoparticles (GM ZnO NPs) at the interface between the gelatin hydrogel and aqueous electrolyte. Diffusion of ammonia through the hydrogel matrices with different gelatin contents induced precipitation of the product in contact with the surface of the aqueous solution of zinc ions. The obtained precipitate was subjected to thermal treatment to partially decompose the adsorbed gelatin in the NP structure. Physicochemical properties of obtained GM ZnO NPs were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), differential thermal analysis (DTA), thermogravimetry (TG), photon correlation spectroscopy (PCS), zeta potential measurements, and inductively coupled plasma-mass spectrometry (ICP-MS). The estimated mean crystallite size of GM ZnO NP powders was in the range from 5.8 to 12.1 nm. The synthesized NPs exhibited nanosheet morphology and arranged into flake-like aggregates. The toxic potential was investigated in vitro in human hepatocellular carcinoma cell line HepG2. The thiazolyl blue tetrazolium bromide (MTS) assay was used to assess cell viability, 2 ',7 '-dichlor-fluorescein-diacetate (DCFH-DA) assay to examine the formation of intracellular reactive oxygen species (ROS), and comet assay to evaluate the genotoxic response. GM ZnO NPs slightly reduced HepG2 cell viability, did not induce ROS formation, and showed low genotoxic potential at very high doses (100 mu g mL(-1)). ZnO NPs fabricated and modified using the proposed methodology deserve further study as potential candidates for antibacterial agents or dietary supplements with low overall toxicity.", journal = "Journal of Nanoparticle Research", title = "Safe-by-design gelatin-modified zinc oxide nanoparticles", number = "9", volume = "23", doi = "10.1007/s11051-021-05312-3" }
Janicijevic, Z., Stankovic, A., Zegura, B., Veljović, Đ., Djekic, L., Krajisnik, D., Filipic, M.,& Stevanovic, M. M.. (2021). Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research, 23(9). https://doi.org/10.1007/s11051-021-05312-3
Janicijevic Z, Stankovic A, Zegura B, Veljović Đ, Djekic L, Krajisnik D, Filipic M, Stevanovic MM. Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research. 2021;23(9). doi:10.1007/s11051-021-05312-3 .
Janicijevic, Zeljko, Stankovic, Ana, Zegura, Bojana, Veljović, Đorđe, Djekic, Ljiljana, Krajisnik, Danina, Filipic, Metka, Stevanovic, Magdalena M., "Safe-by-design gelatin-modified zinc oxide nanoparticles" in Journal of Nanoparticle Research, 23, no. 9 (2021), https://doi.org/10.1007/s11051-021-05312-3 . .