Show simple item record

dc.creatorKnežević, Zorica
dc.creatorBobić, Svetlana
dc.creatorMilutinović, A
dc.creatorObradović, Bojana
dc.creatorMojović, Ljiljana
dc.creatorBugarski, Branko
dc.date.accessioned2021-03-10T10:04:24Z
dc.date.available2021-03-10T10:04:24Z
dc.date.issued2002
dc.identifier.issn1359-5113
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/493
dc.description.abstractLipase from Candida rugosa was immobilized in alginate beads for possible application in non-aqueous or microaqueous reaction systems. An electrostatic droplet generation technique was used for production of small diameter ( lt 1 mm) lipase-alginate beads. This technique provided negligible loss of the lipase (immobilization efficiencies were 98.2-99.2%). Under optimal immobilization conditions (applied potential 4.9 kV, needle gauge 21, 2% sodium alginate solution) the lipase-alginate beads, 0.65 mm in diameter, retained enzyme activity equivalent to 75% that of free lipase. The activity of the immobilized lipase was verified in the reaction of palm oil hydrolysis in a lecithin/isooctane system. The reaction rate with alginate-immobilized lipase was lower than with the free enzyme but the final conversions were approximately the same (similar to 74%). Immobilized lipase could be used for up to three reaction cycles with little loss of activity.en
dc.publisherElsevier Sci Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceProcess Biochemistry
dc.subjectlipaseen
dc.subjectimmobilizationen
dc.subjectalginateen
dc.subjectelectrostatic extrusionen
dc.subjectpalm oil hydrolysisen
dc.titleAlginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane systemen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage318
dc.citation.issue3
dc.citation.other38(3): 313-318
dc.citation.spage313
dc.citation.volume38
dc.identifier.doi10.1016/S0032-9592(02)00085-7
dc.identifier.rcubconv_1928
dc.identifier.scopus2-s2.0-0036842712
dc.identifier.wos000179665400003
dc.type.versionpublishedVersion


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record