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Imobilizacija hromatofora riba radi korišćenja kao biosenzor za biološke toksine

dc.creatorMojović, Ljiljana
dc.creatorJovanović, G.
dc.date.accessioned2021-03-10T10:05:01Z
dc.date.available2021-03-10T10:05:01Z
dc.date.issued2003
dc.identifier.issn1451-9372
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/502
dc.description.abstractChromatophores isolated from the Siamese fighting fish, Betta splendens, represent a class of living cells that provide a vivid color response to microbial pathogens and environmental toxins. The selection of the most appropriate microcarrier and the development of the optimal technique for the chromatophore immobilization in order to enable directed transport of the sensor cells throughout microchannels of the biosensor, as well to preserve the cell survival and its functionality was studied. Microcarriers derived from glass, polystyrene and gelatin (collagen) were tested as substrates for chromatophore attachement. Gelatin microcarriers were found to be the most suitable, due to high attachment efficiency (95% of attached cells), preservation of the cell viability and enhanced cell sensitivity. The optimum conditions for fish cell immobilization on collagen microcarriers were determined based on the cell-to-microcarrier bead ratio and the pH of the solution. The rate of cell attachment to the gelatin microcarrier followed first-order kinetics. Pretreatment of the gelatin beads with fibronectin, known as a cell attachment-promoting agent, resulted in a 10% higher attachment rate constant (k).en
dc.publisherAssociation of the Chemical Engineers of Serbia
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceChemical Industry & Chemical Engineering Quarterly
dc.subjectBetta splendensen
dc.subjectBiosensoren
dc.subjectCell immobilisationen
dc.subjectFish chromatophoresen
dc.subjectGelatin carrieren
dc.titleImmobilization of fish chromatophores for use as a micro-biosensor for biological toxinsen
dc.titleImobilizacija hromatofora riba radi korišćenja kao biosenzor za biološke toksinesr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage610
dc.citation.issue4
dc.citation.other9(4): 605-610
dc.citation.spage605
dc.citation.volume9
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/2223/499.pdf
dc.identifier.pmid
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_technorep_502
dc.identifier.scopus2-s2.0-85034443088
dc.type.versionpublishedVersion


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