Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
Само за регистроване кориснике
1995
Аутори
Sajc, L.Vunjak-Novaković, Gordana
Grubisić, D.
Kovačević, N.
Vuković, Dragan
Bugarski, Branko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intrapar...ticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
Извор:
Applied Microbiology and Biotechnology, 1995, 43, 3, 416-423Издавач:
- Springer, New York
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Sajc, L. AU - Vunjak-Novaković, Gordana AU - Grubisić, D. AU - Kovačević, N. AU - Vuković, Dragan AU - Bugarski, Branko PY - 1995 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/60 AB - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor EP - 423 IS - 3 SP - 416 VL - 43 DO - 10.1007/BF00218443 ER -
@article{ author = "Sajc, L. and Vunjak-Novaković, Gordana and Grubisić, D. and Kovačević, N. and Vuković, Dragan and Bugarski, Branko", year = "1995", abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor", pages = "423-416", number = "3", volume = "43", doi = "10.1007/BF00218443" }
Sajc, L., Vunjak-Novaković, G., Grubisić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology Springer, New York., 43(3), 416-423. https://doi.org/10.1007/BF00218443
Sajc L, Vunjak-Novaković G, Grubisić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423. doi:10.1007/BF00218443 .
Sajc, L., Vunjak-Novaković, Gordana, Grubisić, D., Kovačević, N., Vuković, Dragan, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423, https://doi.org/10.1007/BF00218443 . .