USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses
2016
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Аутори
Miljković, MionaDavidović, Slađana
Nešić, Aleksandra
Mihajlovski, Katarina
Dimitrijević, Suzana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Dextransucrase (DS) is extracellular enzyme that catalyses the synthesis of dextran using
sucrose as a substrate, mainly produced by microorganisms belonging to the genera
Lactobacillus, Leuconostoc and Streptococcus. The aim of this study was usage of byproducts
from sugar refineries, molasses and sugar beet pulp (SBP) as a cheap substrate for
DS production. Fermentation process was performed by a natural isolate from water kefir
grain Leuconostoc mesenteroides T3. Molasses (ethanol Factory Alpis, Kovin, Serbia) with
addition of SBP (Fibrex 620, Nordic Sugar, Denmark) was used as fermentation media.
Molasses was diluted in distilledwater to a concentration of 2.5%. SBP was grounded in a
mortarand pestle to a particle size of 500–800μm. Four different treatments were
performed on the SBP before it was added into molasses: NaOH extraction, enzymatic
pretreatment (Viskozym), biological pretreatment (Paenibacillus chitinolyticus CKS1) and
combination of microwave and ultrasound... treatment. The enzyme activity was determined
by measuring the concentration of released reducing sugars by DNS method at 540nm,
using fructose as a standard.Combination of microwave and ultrasound treatment as well
as biological pretreatment resulted in low DS activity. The best results of 1.45U/ml were
obtained with alkali and enzymatic pretreatments. Considering that enzymatic treatment
is environmental friendly it provides suitable method for SBP pretreatment for enhanced
DS production on molasses.
Извор:
SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE, 2016, 77-Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Miljković, Miona AU - Davidović, Slađana AU - Nešić, Aleksandra AU - Mihajlovski, Katarina AU - Dimitrijević, Suzana PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6241 AB - Dextransucrase (DS) is extracellular enzyme that catalyses the synthesis of dextran using sucrose as a substrate, mainly produced by microorganisms belonging to the genera Lactobacillus, Leuconostoc and Streptococcus. The aim of this study was usage of byproducts from sugar refineries, molasses and sugar beet pulp (SBP) as a cheap substrate for DS production. Fermentation process was performed by a natural isolate from water kefir grain Leuconostoc mesenteroides T3. Molasses (ethanol Factory Alpis, Kovin, Serbia) with addition of SBP (Fibrex 620, Nordic Sugar, Denmark) was used as fermentation media. Molasses was diluted in distilledwater to a concentration of 2.5%. SBP was grounded in a mortarand pestle to a particle size of 500–800μm. Four different treatments were performed on the SBP before it was added into molasses: NaOH extraction, enzymatic pretreatment (Viskozym), biological pretreatment (Paenibacillus chitinolyticus CKS1) and combination of microwave and ultrasound treatment. The enzyme activity was determined by measuring the concentration of released reducing sugars by DNS method at 540nm, using fructose as a standard.Combination of microwave and ultrasound treatment as well as biological pretreatment resulted in low DS activity. The best results of 1.45U/ml were obtained with alkali and enzymatic pretreatments. Considering that enzymatic treatment is environmental friendly it provides suitable method for SBP pretreatment for enhanced DS production on molasses. C3 - SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE T1 - USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses SP - 77 UR - https://hdl.handle.net/21.15107/rcub_technorep_6241 ER -
@conference{ author = "Miljković, Miona and Davidović, Slađana and Nešić, Aleksandra and Mihajlovski, Katarina and Dimitrijević, Suzana", year = "2016", abstract = "Dextransucrase (DS) is extracellular enzyme that catalyses the synthesis of dextran using sucrose as a substrate, mainly produced by microorganisms belonging to the genera Lactobacillus, Leuconostoc and Streptococcus. The aim of this study was usage of byproducts from sugar refineries, molasses and sugar beet pulp (SBP) as a cheap substrate for DS production. Fermentation process was performed by a natural isolate from water kefir grain Leuconostoc mesenteroides T3. Molasses (ethanol Factory Alpis, Kovin, Serbia) with addition of SBP (Fibrex 620, Nordic Sugar, Denmark) was used as fermentation media. Molasses was diluted in distilledwater to a concentration of 2.5%. SBP was grounded in a mortarand pestle to a particle size of 500–800μm. Four different treatments were performed on the SBP before it was added into molasses: NaOH extraction, enzymatic pretreatment (Viskozym), biological pretreatment (Paenibacillus chitinolyticus CKS1) and combination of microwave and ultrasound treatment. The enzyme activity was determined by measuring the concentration of released reducing sugars by DNS method at 540nm, using fructose as a standard.Combination of microwave and ultrasound treatment as well as biological pretreatment resulted in low DS activity. The best results of 1.45U/ml were obtained with alkali and enzymatic pretreatments. Considering that enzymatic treatment is environmental friendly it provides suitable method for SBP pretreatment for enhanced DS production on molasses.", journal = "SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE", title = "USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses", pages = "77", url = "https://hdl.handle.net/21.15107/rcub_technorep_6241" }
Miljković, M., Davidović, S., Nešić, A., Mihajlovski, K.,& Dimitrijević, S.. (2016). USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses. in SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE, 77. https://hdl.handle.net/21.15107/rcub_technorep_6241
Miljković M, Davidović S, Nešić A, Mihajlovski K, Dimitrijević S. USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses. in SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE. 2016;:77. https://hdl.handle.net/21.15107/rcub_technorep_6241 .
Miljković, Miona, Davidović, Slađana, Nešić, Aleksandra, Mihajlovski, Katarina, Dimitrijević, Suzana, "USAGE OF BY-PRODUCTS FROM SUGAR INDUSTRY AS A CHEAP SUBSTRATE FOR DEXTRANSUCRASE PRODUCTION Different treatments of sugar beet pulp for enhanced dextransucrase production on molasses" in SOLID URBAN WASTE MANAGEMENT XXI IUPAC CHEMRAWN CONFERENCE (2016):77, https://hdl.handle.net/21.15107/rcub_technorep_6241 .