The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme
Само за регистроване кориснике
2021
Аутори
Jovanović, AleksandraPetrović, Predrag
Đorđević, Verica
Mijin, Dušan
Zdunić, Gordana
Šavikin, Katarina
Bugarski, Branko
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concent...ration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.
Кључне речи:
antioxidant activity / microwave-assisted extraction / particle size / polyphenols / solvent-to-solid ratioИзвор:
Proceedings / VII International Congress "Engineering, Environment and Materials in Process Industry" EEM2021, Jahorina, March 17-19, 2021, 2021, 137-143Издавач:
- Zvornik : Faculty of Technology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200003 (Институт за проучавање лековитог биља 'Др Јосиф Панчић', Београд) (RS-MESTD-inst-2020-200003)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Jovanović, Aleksandra AU - Petrović, Predrag AU - Đorđević, Verica AU - Mijin, Dušan AU - Zdunić, Gordana AU - Šavikin, Katarina AU - Bugarski, Branko PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6443 AB - Aromatic plants are commercially important and find wide use in food and pharmaceutical industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects. Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total volume of solvent and solid plant material, improving the recovery of polyphenols. In the study, wild thyme herb was evaluated as a source of polyphenols, which were extracted by the application of microwaves instead of the traditional extraction techniques. The objective of the present study was optimization of microwave-assisted extraction process through varying irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0- 60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method) and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle size have not shown statistically significant influence on polyphenol yield, while there were significant differences between extracts obtained by various temperatures and solvent-to-solid ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was no statistically significant difference between ethanol concentration in the range from 30% to 50%. The highest polyphenol yield and antioxidant activity were achived in the extract with particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and 27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be used as an efficient and time saving alternative to traditional extraction techniques for extraction of bioactive polyphenolic compounds from wild thyme. PB - Zvornik : Faculty of Technology C3 - Proceedings / VII International Congress "Engineering, Environment and Materials in Process Industry" EEM2021, Jahorina, March 17-19, 2021 T1 - The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme EP - 143 SP - 137 DO - 10.7251/EEMEN2101137J ER -
@conference{ author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Mijin, Dušan and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko", year = "2021", abstract = "Aromatic plants are commercially important and find wide use in food and pharmaceutical industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects. Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total volume of solvent and solid plant material, improving the recovery of polyphenols. In the study, wild thyme herb was evaluated as a source of polyphenols, which were extracted by the application of microwaves instead of the traditional extraction techniques. The objective of the present study was optimization of microwave-assisted extraction process through varying irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0- 60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method) and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle size have not shown statistically significant influence on polyphenol yield, while there were significant differences between extracts obtained by various temperatures and solvent-to-solid ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was no statistically significant difference between ethanol concentration in the range from 30% to 50%. The highest polyphenol yield and antioxidant activity were achived in the extract with particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and 27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be used as an efficient and time saving alternative to traditional extraction techniques for extraction of bioactive polyphenolic compounds from wild thyme.", publisher = "Zvornik : Faculty of Technology", journal = "Proceedings / VII International Congress "Engineering, Environment and Materials in Process Industry" EEM2021, Jahorina, March 17-19, 2021", title = "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme", pages = "143-137", doi = "10.7251/EEMEN2101137J" }
Jovanović, A., Petrović, P., Đorđević, V., Mijin, D., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in Proceedings / VII International Congress "Engineering, Environment and Materials in Process Industry" EEM2021, Jahorina, March 17-19, 2021 Zvornik : Faculty of Technology., 137-143. https://doi.org/10.7251/EEMEN2101137J
Jovanović A, Petrović P, Đorđević V, Mijin D, Zdunić G, Šavikin K, Bugarski B. The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in Proceedings / VII International Congress "Engineering, Environment and Materials in Process Industry" EEM2021, Jahorina, March 17-19, 2021. 2021;:137-143. doi:10.7251/EEMEN2101137J .
Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Mijin, Dušan, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme" in Proceedings / VII International Congress "Engineering, Environment and Materials in Process Industry" EEM2021, Jahorina, March 17-19, 2021 (2021):137-143, https://doi.org/10.7251/EEMEN2101137J . .