Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis
Само за регистроване кориснике
2022
Аутори
Nešović, KatarinaJanković, Ana
Surudžić, Rade
Vukašinović-Sekulić, Maja
Kojić, Vesna
Radetić, Tamara
Mišković-Stanković, Vesna
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dyna...mic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37 C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.
Извор:
Tissue Engineering Part A, 2022, 28, S1, S-132-Издавач:
- Mary Ann Liebert, Inc.
Напомена:
- Book of Abstracts / 6th World Congress - TERMIS 2021, 15-19 November 2021, Maastricht, The Netherlands
Колекције
Институција/група
Inovacioni centarTY - CONF AU - Nešović, Katarina AU - Janković, Ana AU - Surudžić, Rade AU - Vukašinović-Sekulić, Maja AU - Kojić, Vesna AU - Radetić, Tamara AU - Mišković-Stanković, Vesna PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6761 AB - Recent advances in biomaterials science are increasingly focused on (re)search for next-generation soft tissue regeneration solutions. Hydrogel-based materials are becoming a focal point to address many tissue engineering requirements, possessing exceptional biocompatibility, with tailorable shape, structure and mechanical properties, as well as infinite possibilities drug delivery. The widespread antibiotics use has led to aggravated resistance issues in many bacterial strains, so the focus is increasingly shifted towards alternative antibacterial solutions such as silver nanoparticles (AgNPs). In this work, we have developed novel composite materials based on poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/ chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles obtained through green in situ constant-voltage electrochemical synthesis. The obtained AgNPs’ properties, sizes and size distributions were examined using UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. In vitro swelling and silver release kinetics were monitored in the simulated physiological conditions (pH7.4, 37 C). The obtained release and swelling isotherms were fitted with several theoretical models that helped discern the mechanisms of these processes. Tensile testing was carried out to examine the composites’ mechanical behavior and elasticity – important properties for soft tissue engineering materials. Finally, the excellent antibacterial activity of the obtained silver/ poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against Staphylococcus aureus and Escherichia coli was confirmed quantitatively by colony counting method, and their non-toxicity was verified towards two model fibroblast cell lines (MRC-5 and L929) utilizing the MTT assay. Through thorough characterization, the obtained nanocomposite hydrogel materials were identified as strong candidates for active antibacterial soft tissue engineering materials. PB - Mary Ann Liebert, Inc. C3 - Tissue Engineering Part A T1 - Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis IS - S1 SP - S-132 VL - 28 DO - 10.1089/ten.tea.2022.29025.abstracts ER -
@conference{ author = "Nešović, Katarina and Janković, Ana and Surudžić, Rade and Vukašinović-Sekulić, Maja and Kojić, Vesna and Radetić, Tamara and Mišković-Stanković, Vesna", year = "2022", abstract = "Recent advances in biomaterials science are increasingly focused on (re)search for next-generation soft tissue regeneration solutions. Hydrogel-based materials are becoming a focal point to address many tissue engineering requirements, possessing exceptional biocompatibility, with tailorable shape, structure and mechanical properties, as well as infinite possibilities drug delivery. The widespread antibiotics use has led to aggravated resistance issues in many bacterial strains, so the focus is increasingly shifted towards alternative antibacterial solutions such as silver nanoparticles (AgNPs). In this work, we have developed novel composite materials based on poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/ chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles obtained through green in situ constant-voltage electrochemical synthesis. The obtained AgNPs’ properties, sizes and size distributions were examined using UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. In vitro swelling and silver release kinetics were monitored in the simulated physiological conditions (pH7.4, 37 C). The obtained release and swelling isotherms were fitted with several theoretical models that helped discern the mechanisms of these processes. Tensile testing was carried out to examine the composites’ mechanical behavior and elasticity – important properties for soft tissue engineering materials. Finally, the excellent antibacterial activity of the obtained silver/ poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against Staphylococcus aureus and Escherichia coli was confirmed quantitatively by colony counting method, and their non-toxicity was verified towards two model fibroblast cell lines (MRC-5 and L929) utilizing the MTT assay. Through thorough characterization, the obtained nanocomposite hydrogel materials were identified as strong candidates for active antibacterial soft tissue engineering materials.", publisher = "Mary Ann Liebert, Inc.", journal = "Tissue Engineering Part A", title = "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis", number = "S1", pages = "S-132", volume = "28", doi = "10.1089/ten.tea.2022.29025.abstracts" }
Nešović, K., Janković, A., Surudžić, R., Vukašinović-Sekulić, M., Kojić, V., Radetić, T.,& Mišković-Stanković, V.. (2022). Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A Mary Ann Liebert, Inc.., 28(S1), S-132. https://doi.org/10.1089/ten.tea.2022.29025.abstracts
Nešović K, Janković A, Surudžić R, Vukašinović-Sekulić M, Kojić V, Radetić T, Mišković-Stanković V. Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A. 2022;28(S1):S-132. doi:10.1089/ten.tea.2022.29025.abstracts .
Nešović, Katarina, Janković, Ana, Surudžić, Rade, Vukašinović-Sekulić, Maja, Kojić, Vesna, Radetić, Tamara, Mišković-Stanković, Vesna, "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis" in Tissue Engineering Part A, 28, no. S1 (2022):S-132, https://doi.org/10.1089/ten.tea.2022.29025.abstracts . .