Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds
Authors
Andreu, AliciaĆorović, Marija
Garcia-Sanz, Carla
Santos, A. Sofia
Milivojević, Ana
Ortega-Nieto, Clara
Mateo, Cesar
Bezbradica, Dejan
Palomo, Jose M.
Article (Published version)
Metadata
Show full item recordAbstract
Enzymatic glycosylation is a versatile and sustainable biotechnological approach that plays a pivotal role in the production of bioactive compounds. This process involves the enzymatic transfer of sugar moieties onto various acceptor molecules, such as small molecules, peptides, or proteins, resulting in the synthesis of glycosides. These glycosides often exhibit enhanced bioactivity, improved solubility, and enhanced stability, making them valuable in pharmaceuticals, nutraceuticals, and the food industry. This review explores the diverse enzymatic glycosylation strategies employed in the synthesis of bioactive compounds. It highlights the enzymatic catalysts involved, including glycosyltransferases, glycosidases, glycophosphorylases, and glycosynthases. It considers the advantages and disadvantages of these biocatalysts in the stereoselective and regioselective synthesis of different types of glycosylated molecules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, ...glycoderivatives, glycopeptides, and glycoproteins with a clear focus on food and pharmaceutical chemistry. Furthermore, the review outlines various sources of sugar donors, activated glycosides, and sugar nucleotides, as well as the utilization of engineered enzymes and microorganisms for glycosylation reactions. The advantages of enzymatic glycosylation, including its high regioselectivity, stereoselectivity, and sustainability, are emphasized. Therefore, these approaches combining the use of different catalytic systems, the improvement of tools such as immobilization technology or chemical or genetic modification to improve the glycosylation process, could be useful tools in continuous biotechnological advancements.
Keywords:
enzymes / glycoderivatives / glycosylation / oligosaccharidesSource:
Catalysts, 2023, 13, 10, 1359-Publisher:
- MDPI
Funding / projects:
- Spanish National Research Council (CSIC)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- European Commission, project “Twinning for intensified enzymatic processes for production of prebiotic-containing functional food and bioactive cosmetics” grant no. 101060130, HORIZON-WIDERA-2021-ACCESS-02-01
- COST Action CA18132 (GlycoNanoProbes)
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Andreu, Alicia AU - Ćorović, Marija AU - Garcia-Sanz, Carla AU - Santos, A. Sofia AU - Milivojević, Ana AU - Ortega-Nieto, Clara AU - Mateo, Cesar AU - Bezbradica, Dejan AU - Palomo, Jose M. PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6804 AB - Enzymatic glycosylation is a versatile and sustainable biotechnological approach that plays a pivotal role in the production of bioactive compounds. This process involves the enzymatic transfer of sugar moieties onto various acceptor molecules, such as small molecules, peptides, or proteins, resulting in the synthesis of glycosides. These glycosides often exhibit enhanced bioactivity, improved solubility, and enhanced stability, making them valuable in pharmaceuticals, nutraceuticals, and the food industry. This review explores the diverse enzymatic glycosylation strategies employed in the synthesis of bioactive compounds. It highlights the enzymatic catalysts involved, including glycosyltransferases, glycosidases, glycophosphorylases, and glycosynthases. It considers the advantages and disadvantages of these biocatalysts in the stereoselective and regioselective synthesis of different types of glycosylated molecules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glycopeptides, and glycoproteins with a clear focus on food and pharmaceutical chemistry. Furthermore, the review outlines various sources of sugar donors, activated glycosides, and sugar nucleotides, as well as the utilization of engineered enzymes and microorganisms for glycosylation reactions. The advantages of enzymatic glycosylation, including its high regioselectivity, stereoselectivity, and sustainability, are emphasized. Therefore, these approaches combining the use of different catalytic systems, the improvement of tools such as immobilization technology or chemical or genetic modification to improve the glycosylation process, could be useful tools in continuous biotechnological advancements. PB - MDPI T2 - Catalysts T1 - Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds IS - 10 SP - 1359 VL - 13 DO - 10.3390/catal13101359 ER -
@article{ author = "Andreu, Alicia and Ćorović, Marija and Garcia-Sanz, Carla and Santos, A. Sofia and Milivojević, Ana and Ortega-Nieto, Clara and Mateo, Cesar and Bezbradica, Dejan and Palomo, Jose M.", year = "2023", abstract = "Enzymatic glycosylation is a versatile and sustainable biotechnological approach that plays a pivotal role in the production of bioactive compounds. This process involves the enzymatic transfer of sugar moieties onto various acceptor molecules, such as small molecules, peptides, or proteins, resulting in the synthesis of glycosides. These glycosides often exhibit enhanced bioactivity, improved solubility, and enhanced stability, making them valuable in pharmaceuticals, nutraceuticals, and the food industry. This review explores the diverse enzymatic glycosylation strategies employed in the synthesis of bioactive compounds. It highlights the enzymatic catalysts involved, including glycosyltransferases, glycosidases, glycophosphorylases, and glycosynthases. It considers the advantages and disadvantages of these biocatalysts in the stereoselective and regioselective synthesis of different types of glycosylated molecules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glycopeptides, and glycoproteins with a clear focus on food and pharmaceutical chemistry. Furthermore, the review outlines various sources of sugar donors, activated glycosides, and sugar nucleotides, as well as the utilization of engineered enzymes and microorganisms for glycosylation reactions. The advantages of enzymatic glycosylation, including its high regioselectivity, stereoselectivity, and sustainability, are emphasized. Therefore, these approaches combining the use of different catalytic systems, the improvement of tools such as immobilization technology or chemical or genetic modification to improve the glycosylation process, could be useful tools in continuous biotechnological advancements.", publisher = "MDPI", journal = "Catalysts", title = "Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds", number = "10", pages = "1359", volume = "13", doi = "10.3390/catal13101359" }
Andreu, A., Ćorović, M., Garcia-Sanz, C., Santos, A. S., Milivojević, A., Ortega-Nieto, C., Mateo, C., Bezbradica, D.,& Palomo, J. M.. (2023). Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds. in Catalysts MDPI., 13(10), 1359. https://doi.org/10.3390/catal13101359
Andreu A, Ćorović M, Garcia-Sanz C, Santos AS, Milivojević A, Ortega-Nieto C, Mateo C, Bezbradica D, Palomo JM. Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds. in Catalysts. 2023;13(10):1359. doi:10.3390/catal13101359 .
Andreu, Alicia, Ćorović, Marija, Garcia-Sanz, Carla, Santos, A. Sofia, Milivojević, Ana, Ortega-Nieto, Clara, Mateo, Cesar, Bezbradica, Dejan, Palomo, Jose M., "Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds" in Catalysts, 13, no. 10 (2023):1359, https://doi.org/10.3390/catal13101359 . .