In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives
Autori
Petrov Ivanković, AnjaMilivojević, Ana
Ćorović, Marija
Simović, Milica
Banjanac, Katarina
Jansen, Patrick
Vukoičić, Ana
van den Bogaard, Ellen
Bezbradica, Dejan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxi...dant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.
Ključne reči:
Blackcurrant / Polyphenols / Enzyme-assisted extraction / Cutaneous microbiota / Skin prebioticIzvor:
Chemical and Biological Technologies in Agriculture, 2023, 10, 1, 125-Izdavač:
- Springer Science and Business Media Deutschland GmbH
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- Horizon Europe 2021–2027 research and innovation programme under grant agreement ID 101060130 (TwinPrebioEnz)
Kolekcije
Institucija/grupa
Inovacioni centarTY - JOUR AU - Petrov Ivanković, Anja AU - Milivojević, Ana AU - Ćorović, Marija AU - Simović, Milica AU - Banjanac, Katarina AU - Jansen, Patrick AU - Vukoičić, Ana AU - van den Bogaard, Ellen AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6870 AB - Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation. PB - Springer Science and Business Media Deutschland GmbH T2 - Chemical and Biological Technologies in Agriculture T1 - In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives IS - 1 SP - 125 VL - 10 DO - 10.1186/s40538-023-00502-8 ER -
@article{ author = "Petrov Ivanković, Anja and Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Jansen, Patrick and Vukoičić, Ana and van den Bogaard, Ellen and Bezbradica, Dejan", year = "2023", abstract = "Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.", publisher = "Springer Science and Business Media Deutschland GmbH", journal = "Chemical and Biological Technologies in Agriculture", title = "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives", number = "1", pages = "125", volume = "10", doi = "10.1186/s40538-023-00502-8" }
Petrov Ivanković, A., Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Jansen, P., Vukoičić, A., van den Bogaard, E.,& Bezbradica, D.. (2023). In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture Springer Science and Business Media Deutschland GmbH., 10(1), 125. https://doi.org/10.1186/s40538-023-00502-8
Petrov Ivanković A, Milivojević A, Ćorović M, Simović M, Banjanac K, Jansen P, Vukoičić A, van den Bogaard E, Bezbradica D. In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture. 2023;10(1):125. doi:10.1186/s40538-023-00502-8 .
Petrov Ivanković, Anja, Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Jansen, Patrick, Vukoičić, Ana, van den Bogaard, Ellen, Bezbradica, Dejan, "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives" in Chemical and Biological Technologies in Agriculture, 10, no. 1 (2023):125, https://doi.org/10.1186/s40538-023-00502-8 . .