Antimikrobna aktivnost i citotoksičnost nanočestica na bazi srebra in situ sintetisanih na pamučnoj tkanini primenom ekstrakta lista oraha

Abstract

U ovom radu je ispitana mogućnost in situ sinteze nanočestica na bazi srebra primenom ekstrakta lista oraha, na pamučnoj tkanini prethodno modifikovanoj limunskom kiselinom. Tokom sinteze su formirane sferne nanočestice prečnika oko 60 nm, koje su ravnomerno raspoređene po površini pamučnih vlakana. Prisustvo nanočestica na bazi srebra je obezbedilo odličnu antimikrobnu aktivnost prema bakterijama Staphylococcus aureus i Escherichia coli, kao i kvascu Candida albicans. Tekstilni nanokompozitni materijal nije citotoksičan prema ćelijama zdravih keratinocita kože (HaCaT linija) i zdravih fibroblasta (MRC-5 linija), što omogućava njegovu bezbednu primenu za medicinske potrebe.

This study discusses the possibility to utilize walnut leaf extract for in situ synthesis of silver-based nanoparticles on cotton fabric previously modified with citric acid. Synthetized spherical nanoparticles with an average diameter of 60 nm were evenly distributed over the surface of cotton fibers. The presence of silver-based nanoparticles provided excellent antimicrobial activity against bacteria Staphylococcus aureus and Escherichia coli, and yeast Candida albicans. The textile nanocomposite did not show any cytotoxicity towards healthy skin keratinocytes cells (HaCaT line) and healthy fibroblast cells (MRC-5 line). Thus, it could be considered as a safe for potential medical applications.