The separation of biologically active extracts from chamomile seeds
Аутори
Milovanović, StojaGrzegorczyk, Agnieszka
Świątek, Łukasz
Tyśkiewicz, Katarzyna
Konkol, Marcin
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the... addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals.
Кључне речи:
antimicrobial activity / antioxidant activity / Chamaemelum nobile / Supercritical carbon dioxide extraction / Cytotoxic activityИзвор:
Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain, 2023Издавач:
- Valladolid : The lnstitute of Bioeconomy of University of Valladolid
Финансирање / пројекти:
- COST Action CA18224 "Green Chemical Engineering Network towards upscaling sustainable processes" (GREENERING) supported by COST (European Cooperation in Science and Technology)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Milovanović, Stoja AU - Grzegorczyk, Agnieszka AU - Świątek, Łukasz AU - Tyśkiewicz, Katarzyna AU - Konkol, Marcin PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6894 AB - The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals. PB - Valladolid : The lnstitute of Bioeconomy of University of Valladolid C3 - Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain T1 - The separation of biologically active extracts from chamomile seeds UR - https://hdl.handle.net/21.15107/rcub_technorep_6894 ER -
@conference{ author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Tyśkiewicz, Katarzyna and Konkol, Marcin", year = "2023", abstract = "The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals.", publisher = "Valladolid : The lnstitute of Bioeconomy of University of Valladolid", journal = "Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain", title = "The separation of biologically active extracts from chamomile seeds", url = "https://hdl.handle.net/21.15107/rcub_technorep_6894" }
Milovanović, S., Grzegorczyk, A., Świątek, Ł., Tyśkiewicz, K.,& Konkol, M.. (2023). The separation of biologically active extracts from chamomile seeds. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain Valladolid : The lnstitute of Bioeconomy of University of Valladolid.. https://hdl.handle.net/21.15107/rcub_technorep_6894
Milovanović S, Grzegorczyk A, Świątek Ł, Tyśkiewicz K, Konkol M. The separation of biologically active extracts from chamomile seeds. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain. 2023;. https://hdl.handle.net/21.15107/rcub_technorep_6894 .
Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Tyśkiewicz, Katarzyna, Konkol, Marcin, "The separation of biologically active extracts from chamomile seeds" in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain (2023), https://hdl.handle.net/21.15107/rcub_technorep_6894 .