BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Development of novel biomaterials for potential biomedical applications requires comprehensive preclinical studies. Traditional methods for biomaterial evaluation are based on in vitro testing routinely performed in monolayer cell cultures followed by in vivo animal studies. However, these methods have numerous limitations. Although studies in cell monolayers allow rapid evaluation of biomaterials by standardized protocols, quantitative and comparable results, cell metabolism and morphology is changed in the 2-dimensional environment often leading to unreliable results. On other hand, animal studies are complex, time-consuming, expensive and raise ethical concerns. One of the approaches to address this problem and obtain reliable results in a more efficient way is utilization of biomimetic bioreactors. These bioreactors are primarily developed as an essential component in tissue engineering mimicking physiological in vivo conditions in particular tissue or organ by providing all necess...ary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) for cell differentiation and metabolic activity. Some examples include perfusion bioreactors, bioreactors with shear stresses and/or dynamic compression, and bioreactor with stretch and shear stresses imitating conditions in vascularized tissues, articular cartilage, and vascular grafts, respectively. Such physiologically relevant, while strictly controlled environment is also beneficial for biomaterial assessment, investigation of cell-biomaterial interactions, and prediction of biomaterial behaviour upon application. The present review provides readers with up-to-date studies and results regarding utilization of biomimetic bioreactors as tools for comprehensive and efficient evaluation of novel biomaterials, such as determination of mechanical characteristics, release of bioactive substances, cell-biomaterial interactions and cytotoxicity.
Кључне речи:
In vivo-like conditions / cytotoxicity / mechanical characterization / release studiesИзвор:
Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia, 2022, 17-Издавач:
- Belgrade : Innovation Center of Faculty of Mechanical Engineering
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- European Union’s Horizon 2020 research and innovation programme (GA no 952033)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Zvicer, Jovana AU - Stojkovska, Jasmina AU - Obradović, Bojana PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6958 AB - Development of novel biomaterials for potential biomedical applications requires comprehensive preclinical studies. Traditional methods for biomaterial evaluation are based on in vitro testing routinely performed in monolayer cell cultures followed by in vivo animal studies. However, these methods have numerous limitations. Although studies in cell monolayers allow rapid evaluation of biomaterials by standardized protocols, quantitative and comparable results, cell metabolism and morphology is changed in the 2-dimensional environment often leading to unreliable results. On other hand, animal studies are complex, time-consuming, expensive and raise ethical concerns. One of the approaches to address this problem and obtain reliable results in a more efficient way is utilization of biomimetic bioreactors. These bioreactors are primarily developed as an essential component in tissue engineering mimicking physiological in vivo conditions in particular tissue or organ by providing all necessary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) for cell differentiation and metabolic activity. Some examples include perfusion bioreactors, bioreactors with shear stresses and/or dynamic compression, and bioreactor with stretch and shear stresses imitating conditions in vascularized tissues, articular cartilage, and vascular grafts, respectively. Such physiologically relevant, while strictly controlled environment is also beneficial for biomaterial assessment, investigation of cell-biomaterial interactions, and prediction of biomaterial behaviour upon application. The present review provides readers with up-to-date studies and results regarding utilization of biomimetic bioreactors as tools for comprehensive and efficient evaluation of novel biomaterials, such as determination of mechanical characteristics, release of bioactive substances, cell-biomaterial interactions and cytotoxicity. PB - Belgrade : Innovation Center of Faculty of Mechanical Engineering C3 - Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia T1 - BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS SP - 17 UR - https://hdl.handle.net/21.15107/rcub_technorep_6958 ER -
@conference{ author = "Zvicer, Jovana and Stojkovska, Jasmina and Obradović, Bojana", year = "2022", abstract = "Development of novel biomaterials for potential biomedical applications requires comprehensive preclinical studies. Traditional methods for biomaterial evaluation are based on in vitro testing routinely performed in monolayer cell cultures followed by in vivo animal studies. However, these methods have numerous limitations. Although studies in cell monolayers allow rapid evaluation of biomaterials by standardized protocols, quantitative and comparable results, cell metabolism and morphology is changed in the 2-dimensional environment often leading to unreliable results. On other hand, animal studies are complex, time-consuming, expensive and raise ethical concerns. One of the approaches to address this problem and obtain reliable results in a more efficient way is utilization of biomimetic bioreactors. These bioreactors are primarily developed as an essential component in tissue engineering mimicking physiological in vivo conditions in particular tissue or organ by providing all necessary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) for cell differentiation and metabolic activity. Some examples include perfusion bioreactors, bioreactors with shear stresses and/or dynamic compression, and bioreactor with stretch and shear stresses imitating conditions in vascularized tissues, articular cartilage, and vascular grafts, respectively. Such physiologically relevant, while strictly controlled environment is also beneficial for biomaterial assessment, investigation of cell-biomaterial interactions, and prediction of biomaterial behaviour upon application. The present review provides readers with up-to-date studies and results regarding utilization of biomimetic bioreactors as tools for comprehensive and efficient evaluation of novel biomaterials, such as determination of mechanical characteristics, release of bioactive substances, cell-biomaterial interactions and cytotoxicity.", publisher = "Belgrade : Innovation Center of Faculty of Mechanical Engineering", journal = "Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia", title = "BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS", pages = "17", url = "https://hdl.handle.net/21.15107/rcub_technorep_6958" }
Zvicer, J., Stojkovska, J.,& Obradović, B.. (2022). BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS. in Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia Belgrade : Innovation Center of Faculty of Mechanical Engineering., 17. https://hdl.handle.net/21.15107/rcub_technorep_6958
Zvicer J, Stojkovska J, Obradović B. BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS. in Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia. 2022;:17. https://hdl.handle.net/21.15107/rcub_technorep_6958 .
Zvicer, Jovana, Stojkovska, Jasmina, Obradović, Bojana, "BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS" in Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia (2022):17, https://hdl.handle.net/21.15107/rcub_technorep_6958 .