dc.creator | Ortega-Nieto, Clara | |
dc.creator | Losada-Gracia, Noelia | |
dc.creator | Vukoičić, Ana | |
dc.creator | Milivojević, Ana | |
dc.creator | Bezbradica, Dejan | |
dc.creator | Palomo, Jose M. | |
dc.date.accessioned | 2023-12-14T07:59:39Z | |
dc.date.available | 2023-12-14T07:59:39Z | |
dc.date.issued | 2023 | |
dc.identifier.isbn | 978-86-7401-389-2 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6959 | |
dc.description.abstract | In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade : University, Faculty of Technology and Metallurgy | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS// | sr |
dc.relation | Horizon Europe 2021-2027 research and innovation programme under grant agreement ID 101060130 (TwinPrebioEnz) | sr |
dc.rights | restrictedAccess | sr |
dc.source | Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade | sr |
dc.subject | metalloenzymes | en |
dc.subject | copper | |
dc.subject | silver | |
dc.subject | phloridzin | |
dc.subject | lacasse-like activity | |
dc.title | NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.citation.spage | 39 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_technorep_6959 | |
dc.type.version | publishedVersion | sr |