Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach
Само за регистроване кориснике
2023
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinat...ions of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.
Извор:
Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023, 2023, 135-Издавач:
- Budapest : University of Technology and Economics
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- COST Action CA18224 "Green Chemical Engineering Network towards upscaling sustainable processes" (GREENERING) supported by COST (European Cooperation in Science and Technology)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Lukić, Ivana AU - Stamenić, Marko AU - Tadić, Vanja AU - Skala, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6984 AB - The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment. In the present study, the preliminary analysis was performed to determine the influence of different scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD. Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant). The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables. PB - Budapest : University of Technology and Economics C3 - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 T1 - Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach SP - 135 UR - https://hdl.handle.net/21.15107/rcub_technorep_6984 ER -
@conference{ author = "Lukić, Ivana and Stamenić, Marko and Tadić, Vanja and Skala, Dejan", year = "2023", abstract = "The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment. In the present study, the preliminary analysis was performed to determine the influence of different scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD. Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant). The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.", publisher = "Budapest : University of Technology and Economics", journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023", title = "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach", pages = "135", url = "https://hdl.handle.net/21.15107/rcub_technorep_6984" }
Lukić, I., Stamenić, M., Tadić, V.,& Skala, D.. (2023). Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 Budapest : University of Technology and Economics., 135. https://hdl.handle.net/21.15107/rcub_technorep_6984
Lukić I, Stamenić M, Tadić V, Skala D. Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:135. https://hdl.handle.net/21.15107/rcub_technorep_6984 .
Lukić, Ivana, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):135, https://hdl.handle.net/21.15107/rcub_technorep_6984 .