Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface proper...ties, cell concentration, viability, and differentiation.
Keywords:
alginate / bone marrow stromal cells / tissue cultivation
Source:
Materials Science Forum, 2005, 494, 525-530
Publisher:
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
TY - JOUR
AU - Bugarski, Diana
AU - Obradović, B
AU - Petakov, M
AU - Jovcić, G
AU - Stojanović, N
AU - Bugarski, Branko
PY - 2005
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/712
AB - Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation.
PB - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
T2 - Materials Science Forum
T1 - Alginate microbeads as potential support for cultivation of bone marrow stromal cells
EP - 530
SP - 525
VL - 494
DO - 10.4028/0-87849-971-7.525
ER -
@article{
author = "Bugarski, Diana and Obradović, B and Petakov, M and Jovcić, G and Stojanović, N and Bugarski, Branko",
year = "2005",
url = "http://TechnoRep.tmf.bg.ac.rs/handle/123456789/712",
abstract = "Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation.",
publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes",
journal = "Materials Science Forum",
title = "Alginate microbeads as potential support for cultivation of bone marrow stromal cells",
pages = "530-525",
volume = "494",
doi = "10.4028/0-87849-971-7.525"
}
Bugarski, D., Obradović, B., Petakov, M., Jovcić, G., Stojanović, N.,& Bugarski, B. (2005). Alginate microbeads as potential support for cultivation of bone marrow stromal cells.
Materials Science Forum
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 525-530.
https://doi.org/10.4028/0-87849-971-7.525
Bugarski D, Obradović B, Petakov M, Jovcić G, Stojanović N, Bugarski B. Alginate microbeads as potential support for cultivation of bone marrow stromal cells. Materials Science Forum. 2005;494:525-530
Bugarski Diana, Obradović B, Petakov M, Jovcić G, Stojanović N, Bugarski Branko, "Alginate microbeads as potential support for cultivation of bone marrow stromal cells" Materials Science Forum, 494 (2005):525-530,
https://doi.org/10.4028/0-87849-971-7.525 .
