Alginate microbeads as potential support for cultivation of bone marrow stromal cells
Apstrakt
Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface proper...ties, cell concentration, viability, and differentiation.
Ključne reči:
alginate / bone marrow stromal cells / tissue cultivationIzvor:
Materials Science Forum, 2005, 494, 525-530Izdavač:
- 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
DOI: 10.4028/0-87849-971-7.525
ISSN: 0255-5476
PubMed:
WoS: 000230985800086
Scopus: 2-s2.0-35248894817
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Bugarski, Diana AU - Obradović, B. AU - Petakov, M AU - Jovcić, G AU - Stojanović, N AU - Bugarski, Branko PY - 2005 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/712 AB - Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation. PB - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes T2 - Materials Science Forum T1 - Alginate microbeads as potential support for cultivation of bone marrow stromal cells EP - 530 SP - 525 VL - 494 DO - 10.4028/0-87849-971-7.525 ER -
@article{ author = "Bugarski, Diana and Obradović, B. and Petakov, M and Jovcić, G and Stojanović, N and Bugarski, Branko", year = "2005", abstract = "Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation.", publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes", journal = "Materials Science Forum", title = "Alginate microbeads as potential support for cultivation of bone marrow stromal cells", pages = "530-525", volume = "494", doi = "10.4028/0-87849-971-7.525" }
Bugarski, D., Obradović, B., Petakov, M., Jovcić, G., Stojanović, N.,& Bugarski, B.. (2005). Alginate microbeads as potential support for cultivation of bone marrow stromal cells. in Materials Science Forum 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 525-530. https://doi.org/10.4028/0-87849-971-7.525
Bugarski D, Obradović B, Petakov M, Jovcić G, Stojanović N, Bugarski B. Alginate microbeads as potential support for cultivation of bone marrow stromal cells. in Materials Science Forum. 2005;494:525-530. doi:10.4028/0-87849-971-7.525 .
Bugarski, Diana, Obradović, B., Petakov, M, Jovcić, G, Stojanović, N, Bugarski, Branko, "Alginate microbeads as potential support for cultivation of bone marrow stromal cells" in Materials Science Forum, 494 (2005):525-530, https://doi.org/10.4028/0-87849-971-7.525 . .