Show simple item record

dc.creatorBugarski, Diana
dc.creatorObradović, B
dc.creatorPetakov, M
dc.creatorJovcić, G
dc.creatorStojanović, N
dc.creatorBugarski, Branko
dc.date.accessioned2021-03-10T10:18:42Z
dc.date.available2021-03-10T10:18:42Z
dc.date.issued2005
dc.identifier.issn0255-5476
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/712
dc.description.abstractAlginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation.en
dc.publisher6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
dc.rightsrestrictedAccess
dc.sourceMaterials Science Forum
dc.subjectalginateen
dc.subjectbone marrow stromal cellsen
dc.subjecttissue cultivationen
dc.titleAlginate microbeads as potential support for cultivation of bone marrow stromal cellsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage530
dc.citation.other494: 525-530
dc.citation.rankM23
dc.citation.spage525
dc.citation.volume494
dc.identifier.doi10.4028/0-87849-971-7.525
dc.identifier.pmid
dc.identifier.rcubconv_6970
dc.identifier.scopus2-s2.0-35248894817
dc.identifier.wos000230985800086
dc.type.versionpublishedVersion


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record