Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain
Само за регистроване кориснике
2024
Аутори
Ilić, NevenaDavidović, Slađana
Milić, Marija
Lađarević, Jelena
Onjia, Antonije
Dimitrijević-Branković, Suzana
Mihajlovski, Katarina
Чланак у часопису (Објављена верзија)
,
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024
Метаподаци
Приказ свих података о документуАпстракт
This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99....0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.
Кључне речи:
White rot fungi / Coriolopsis trogii / Laccase / Azo dye / Decolorization / Immobilization / Brewerʼs spent grain / ReuseИзвор:
Environmental Science and Pollution Research, 2024Издавач:
- Springer
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- National Project, Proof of Concept, Innovation Fund, Republic of Serbia, PoC5634 "Green biocatalyst for decolorization and degradation of azo dyes from industrial wastewater: a white-rot fungal laccase immobilized on recycled agro-industrial waste”
Колекције
Институција/група
Inovacioni centarTY - JOUR AU - Ilić, Nevena AU - Davidović, Slađana AU - Milić, Marija AU - Lađarević, Jelena AU - Onjia, Antonije AU - Dimitrijević-Branković, Suzana AU - Mihajlovski, Katarina PY - 2024 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7444 AB - This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental disposal. PB - Springer T2 - Environmental Science and Pollution Research T1 - Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain DO - 10.1007/s11356-024-33367-x ER -
@article{ author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina", year = "2024", abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental disposal.", publisher = "Springer", journal = "Environmental Science and Pollution Research", title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain", doi = "10.1007/s11356-024-33367-x" }
Ilić, N., Davidović, S., Milić, M., Lađarević, J., Onjia, A., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2024). Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research Springer.. https://doi.org/10.1007/s11356-024-33367-x
Ilić N, Davidović S, Milić M, Lađarević J, Onjia A, Dimitrijević-Branković S, Mihajlovski K. Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research. 2024;. doi:10.1007/s11356-024-33367-x .
Ilić, Nevena, Davidović, Slađana, Milić, Marija, Lađarević, Jelena, Onjia, Antonije, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain" in Environmental Science and Pollution Research (2024), https://doi.org/10.1007/s11356-024-33367-x . .