A novel source of biologically active compounds – The leaves of Serbian herbaceous peonies
Аутори
Batinić, PetarJovanović, Aleksandra
Stojković, Dejan
Čutović, Natalija
Cvijetić, Ilija
Gašić, Uroš
Carević, Tamara
Zengin, Gökhan
Marinković, Aleksandar
Marković, Tatjana
Метаподаци
Приказ свих података о документуАпстракт
In order to gain further insight into how various extraction techniques (maceration, microwave-, and ultrasound-assisted extractions) affect the chemical profile and biological activities of leaf extracts from Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L., this research was performed. The targeted chemical characterization of the extracts was achieved using the Ultra-High-Performance-Liquid-Chromatography-Linear-Trap-Mass-Spectrometry OrbiTrap instrumental technique, while Fourier Transform Infrared Spectroscopy was conducted to investigate the structural properties of the examined leaf extracts. According to the results, the species P. officinalis, Božurna locality as the origin of the plant material, and microwave-assisted extraction produced the maximum polyphenol yield, (491.9 ± 2.7 mg gallic acid equivalent (GAE)/mL). The ethanolic extracts exhibited moderate antioxidant activity as evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and phosphomolybdenu...m tests. With MIC values of 0.125 mg/mL, the leaf extracts produced by ultrasound-assisted extraction and maceration (Deliblato sands and Bogovo gumno) had the best antibacterial activity against Pseudomonas aeruginosa and Salmonella Typhimurium. Ultrasound-assisted extraction has proven to produce the most effective antimicrobial agents. Inhibitory potential towards glucosidase, amylase, cholinesterases, and tyrosinase was evaluated in enzyme inhibition assays and molecular docking simulations. Results show that leaves of P. tenuifolia L. obtained by ultrasound-assisted extraction had the highest acetylcholinesterase and butyrylcholinesterase inhibitory activity. Namely, the complexity of the polyphenol structures, the extraction method, the used locality, and the different mechanisms of the reactions between bioactives from leaf extracts and other components (free radicals, microorganisms, and enzymes) are the main factors that influence the results of the antioxidant tests, as well as the antibacterial and enzyme-inhibitory activities of the extracts. Hydroxymethyl-phenyl pentosyl-hexoside and acetyl-hydroxyphenyl-hexoside were the first time identified in the leaf extract of the Paeonia species. Due to their proven biological activities and the confirmed existence of bioactive compounds, leaf extracts may find use in foodstuffs, functional foods, and pharmaceutical products.
Кључне речи:
Antimicrobial potential / Balkan peony / Chemical profile / Common peony / Enzyme inhibitors / Steppe peony / Total antioxidant capacityИзвор:
Saudi Pharmaceutical Journal, 06-2024, 32, 6, 102090-Издавач:
- Elsevier B.V.
Финансирање / пројекти:
- Ministry of Science, Technological Development, and Innovation of the Republic of Serbia (Grants 451-03- 1202/2021-09/3)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200003 (Институт за проучавање лековитог биља 'Др Јосиф Панчић', Београд) (RS-MESTD-inst-2020-200003)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
Институција/група
Tehnološko-metalurški fakultetBatinić, P., Jovanović, A., Stojković, D., Čutović, N., Cvijetić, I., Gašić, U., Carević, T., Zengin, G., Marinković, A.,& Marković, T.. (2024-06). A novel source of biologically active compounds – The leaves of Serbian herbaceous peonies. in Saudi Pharmaceutical Journal Elsevier B.V.., 32(6), 102090. https://doi.org/10.1016/j.jsps.2024.102090
Batinić P, Jovanović A, Stojković D, Čutović N, Cvijetić I, Gašić U, Carević T, Zengin G, Marinković A, Marković T. A novel source of biologically active compounds – The leaves of Serbian herbaceous peonies. in Saudi Pharmaceutical Journal. 2024;32(6):102090. doi:10.1016/j.jsps.2024.102090 .
Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Čutović, Natalija, Cvijetić, Ilija, Gašić, Uroš, Carević, Tamara, Zengin, Gökhan, Marinković, Aleksandar, Marković, Tatjana, "A novel source of biologically active compounds – The leaves of Serbian herbaceous peonies" in Saudi Pharmaceutical Journal, 32, no. 6 (2024-06):102090, https://doi.org/10.1016/j.jsps.2024.102090 . .