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Bioethanol from corn meal hydrolyzates

Proizvodnja bioetanola na hidrolizovanom kukuruznom brašnu

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2005
1451-93720504189N.pdf (398.9Kb)
Authors
Nikolić, Svetlana
Rakin, Marica
Vukašinović-Sekulić, Maja
Šiler-Marinković, Slavica
Mojović, Ljiljana
Article (Published version)
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Abstract
The two-step enzymatic hydrolysis of corn meal by commercially available α-amylase and amyloglycosidase and the subsequent or simultaneous ethanol fermentation of the hydrolyzates by Saccharomyces cerevisiae yeast were studied. The conditions of starch hydrolysis, such as substrate and enzyme concentration and the time required for enzymatic action, were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The corn meal hydrolyzates obtained were good substrates for ethanol fermentation by Saccharomyces cerevisiae. A yield of ethanol of more than 80% of the theoretical one was achieved with a satisfactory product to substrate yield Yp/s (g/g) and good ethanol volumetric productivity P (g/lh). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. Savings in time and energy could be realized by such a process.
U radu je ispitivan postupak dvojno enzimske hidrolize kukuruznog brašna pomoću komercijalnih enzima α-amilaze i amiloglukozidaze i naknadna ili uporedna fermentacija hidrolizata pomoću kvasca Saccharomyces cerevisiae. Uslovi hidrolize kao što su koncentracija supstrata i enzima i vreme dejstva pojedinih enzima su optimizovani sa aspekta oba procesa (i fermentacije i hidrolize). Dobijeni hidrolizati kukuruznog brašna su se pokazali kao pogodni supstrati za etanolnu fermentaciju pomoću kvasca Saccharomyces cerevisiae pri čemu je ostvaren prinos etanola od više od 80% od teorijskog prinosa i sa zadovoljavajućim vrednostima prinosa produkta po substratu Yp/s (g/g) i volumetrijske produktivnoosti P (g/lh). U toku postupka sa uporednim odvijanjem hidrolize i fermentacije nije primećen nedostatak fermentabilnih šećera. Osim toga ovim postupkom moguće je skratiti ukupno vreme procesa i ostvariti određene energetske uštede.
Keywords:
enzyme hydrolysis / starch / corn / ethanol fermentation / enzimska hidroliza / skrob / kukuruz / alkoholna fermentacija
Source:
Chemical Industry & Chemical Engineering Quarterly, 2005, 11, 4, 189-194
Publisher:
  • Association of the Chemical Engineers of Serbia
Funding / projects:
  • Projekat Ministarstva nauke Republike Srbije, br. TD 7042B

DOI: 10.2298/CICEQ0504189N

ISSN: 1451-9372

Scopus: 2-s2.0-85034445899
[ Google Scholar ]
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/755
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Šiler-Marinković, Slavica
AU  - Mojović, Ljiljana
PY  - 2005
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/755
AB  - The two-step enzymatic hydrolysis of corn meal by commercially available α-amylase and amyloglycosidase and the subsequent or simultaneous ethanol fermentation of the hydrolyzates by Saccharomyces cerevisiae yeast were studied. The conditions of starch hydrolysis, such as substrate and enzyme concentration and the time required for enzymatic action, were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The corn meal hydrolyzates obtained were good substrates for ethanol fermentation by Saccharomyces cerevisiae. A yield of ethanol of more than 80% of the theoretical one was achieved with a satisfactory product to substrate yield Yp/s (g/g) and good ethanol volumetric productivity P (g/lh). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. Savings in time and energy could be realized by such a process.
AB  - U radu je ispitivan postupak dvojno enzimske hidrolize kukuruznog brašna pomoću komercijalnih enzima α-amilaze i amiloglukozidaze i naknadna ili uporedna fermentacija hidrolizata pomoću kvasca Saccharomyces cerevisiae. Uslovi hidrolize kao što su koncentracija supstrata i enzima i vreme dejstva pojedinih enzima su optimizovani sa aspekta oba procesa (i fermentacije i hidrolize). Dobijeni hidrolizati kukuruznog brašna su se pokazali kao pogodni supstrati za etanolnu fermentaciju pomoću kvasca Saccharomyces cerevisiae pri čemu je ostvaren prinos etanola od više od 80% od teorijskog prinosa i sa zadovoljavajućim vrednostima prinosa produkta po substratu Yp/s (g/g) i volumetrijske produktivnoosti P (g/lh). U toku postupka sa uporednim odvijanjem hidrolize i fermentacije nije primećen nedostatak fermentabilnih šećera. Osim toga ovim postupkom moguće je skratiti ukupno vreme procesa i ostvariti određene energetske uštede.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Bioethanol from corn meal hydrolyzates
T1  - Proizvodnja bioetanola na hidrolizovanom kukuruznom brašnu
EP  - 194
IS  - 4
SP  - 189
VL  - 11
DO  - 10.2298/CICEQ0504189N
UR  - conv_1628
ER  - 
@article{
author = "Nikolić, Svetlana and Rakin, Marica and Vukašinović-Sekulić, Maja and Šiler-Marinković, Slavica and Mojović, Ljiljana",
year = "2005",
abstract = "The two-step enzymatic hydrolysis of corn meal by commercially available α-amylase and amyloglycosidase and the subsequent or simultaneous ethanol fermentation of the hydrolyzates by Saccharomyces cerevisiae yeast were studied. The conditions of starch hydrolysis, such as substrate and enzyme concentration and the time required for enzymatic action, were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The corn meal hydrolyzates obtained were good substrates for ethanol fermentation by Saccharomyces cerevisiae. A yield of ethanol of more than 80% of the theoretical one was achieved with a satisfactory product to substrate yield Yp/s (g/g) and good ethanol volumetric productivity P (g/lh). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. Savings in time and energy could be realized by such a process., U radu je ispitivan postupak dvojno enzimske hidrolize kukuruznog brašna pomoću komercijalnih enzima α-amilaze i amiloglukozidaze i naknadna ili uporedna fermentacija hidrolizata pomoću kvasca Saccharomyces cerevisiae. Uslovi hidrolize kao što su koncentracija supstrata i enzima i vreme dejstva pojedinih enzima su optimizovani sa aspekta oba procesa (i fermentacije i hidrolize). Dobijeni hidrolizati kukuruznog brašna su se pokazali kao pogodni supstrati za etanolnu fermentaciju pomoću kvasca Saccharomyces cerevisiae pri čemu je ostvaren prinos etanola od više od 80% od teorijskog prinosa i sa zadovoljavajućim vrednostima prinosa produkta po substratu Yp/s (g/g) i volumetrijske produktivnoosti P (g/lh). U toku postupka sa uporednim odvijanjem hidrolize i fermentacije nije primećen nedostatak fermentabilnih šećera. Osim toga ovim postupkom moguće je skratiti ukupno vreme procesa i ostvariti određene energetske uštede.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Bioethanol from corn meal hydrolyzates, Proizvodnja bioetanola na hidrolizovanom kukuruznom brašnu",
pages = "194-189",
number = "4",
volume = "11",
doi = "10.2298/CICEQ0504189N",
url = "conv_1628"
}
Nikolić, S., Rakin, M., Vukašinović-Sekulić, M., Šiler-Marinković, S.,& Mojović, L.. (2005). Bioethanol from corn meal hydrolyzates. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 11(4), 189-194.
https://doi.org/10.2298/CICEQ0504189N
conv_1628
Nikolić S, Rakin M, Vukašinović-Sekulić M, Šiler-Marinković S, Mojović L. Bioethanol from corn meal hydrolyzates. in Chemical Industry & Chemical Engineering Quarterly. 2005;11(4):189-194.
doi:10.2298/CICEQ0504189N
conv_1628 .
Nikolić, Svetlana, Rakin, Marica, Vukašinović-Sekulić, Maja, Šiler-Marinković, Slavica, Mojović, Ljiljana, "Bioethanol from corn meal hydrolyzates" in Chemical Industry & Chemical Engineering Quarterly, 11, no. 4 (2005):189-194,
https://doi.org/10.2298/CICEQ0504189N .,
conv_1628 .

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