Some aspects of the quantitative analysis of cyclic nucleotides by fast-atom bombardment mass spectrometry

Abstract

The possibilities of the quantitative analysis of cyclic nucleotides by fast-atom bombardment are examined. Aspects such as the amount of matrix, the formation of protonatcd ion species and the presence of more than one cyclic nucleotide in the sample are discussed and the conclusion has been reached that these aspects are in direct relation with the quantification of the data obtained from the mass spectra. Consideration of these aspects suggests that fast-atom bombardment docs not seem to be a convenient ionisation technique for the quantitative analysis of cyclic nucleotides.