The retention behavior of some uracil derivatives in normal and reversed-phase chromatography. Lipophilicity of the compounds

Abstract

The chromatographic behavior of newly synthesized uracil derivatives has been studied by normal and reversed-phase thin-layer chromatography. Benzene-methanol, benzene-acetonitrile, and benzene-isopropanol were used as mobile phases in normal-phase chromatography and water-methanol and water-acetonitrile in reversed-phase chromatography. The mechanism of retention on different TLC supports was investigated. The retention constants determined for the uracils are discussed in terms of the physicochemical properties of both the solutes and stationary and mobile phases. R-M(0) values derived by extrapolation from reversed-phase chromatography were correlated with the hydrophobicity log P calculated by use of the commercial program ACD/logP. In normal-phase chromatography values for C-0 (= R-M(0/n)) were correlated with the hydrophobicity log P. Good correlation was obtained for both types of chromatography. Retention constants R-M(0) and C-0 can be used to express the lipophilicity of the compounds investigated.