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dc.creatorManojlović, V.
dc.creatorĐonlagić, Jasna
dc.creatorObradović, Bojana
dc.creatorNedović, Viktor
dc.creatorBugarski, Branko
dc.date.accessioned2021-03-10T10:32:09Z
dc.date.available2021-03-10T10:32:09Z
dc.date.issued2006
dc.identifier.issn1178-2013
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/917
dc.description.abstractThe process of electrostatic extrusion as a method for cell immobilization was investigated that could be used for potential applications in medicine. An attempt was made to assess the effects of cell addition and polymer concentration on the overall entrapment procedure, ie, on each stage of immobilization: polymer-cell suspension rheological characteristics, electrostatic extrusion process, and the process of gelation. The findings should contribute to a better understanding of polymer-cell interactions, which could be crucial in possible medical treatments. Alginate-yeast was used as a model system for carrier-cells. The electrostatic extrusion was considered as a complex two-phase flow system and the effects of cell and alginate concentrations on the resulting microbead size and uniformity were assessed. Under investigated conditions, microbeads 50-600 mu m in diameter were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with higher standard deviations in size. We attempted to rationalize the findings by rheological characterization of the cell-alginate suspensions. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5x10(8) and 1x10(9) cells/mL) did not significantly affect the rheological properties of Na-alginate solution. Lastly, we investigated the kinetics of alginate gelation with respect to the quantity of Ca2+ ions and cell presence. The gelation kinetics were examined under conditions of limited supply with Ca2+ ions, which can be essential for immobilization of highly sensitive mammalian cells that require minimal exposure to CaCl2 solution. The molar ratio of G units to Ca2+ ions of 3.8:1 provided complete crosslinking, while the increase in alginate concentration resulted in prolonged gelation times but higher strength of the resulting gel. The cell presence decreased the rate of network formation as well as the strength of the obtained Ca-alginate hydrogel.en
dc.publisherDove Medical Press Ltd, Albany
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceInternational Journal of Nanomedicine
dc.subjectalginateen
dc.subjectelectrostatic extrusionen
dc.subjectrheologyen
dc.subjectyeast cellsen
dc.titleImmobilization of cells by electrostatic droplet generation: a model system for potential application in medicineen
dc.typearticle
dc.rights.licenseBY-NC
dc.citation.epage171
dc.citation.issue2
dc.citation.other1(2): 163-171
dc.citation.spage163
dc.citation.volume1
dc.identifier.doi10.2147/nano.2006.1.2.163
dc.identifier.pmid17722532
dc.identifier.rcubconv_2933
dc.identifier.scopus2-s2.0-34548608481
dc.identifier.wos000252047700006
dc.type.versionpublishedVersion


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