Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies
Apstrakt
In this study, the process of electrostatic extrusion as a method for cell immobilization was investigated. We have assessed the effects of concentrations of yeast cells (as a model cell type) and Na alginate on the size of the resulting microbeads and attempted to rationalize the obtained findings by rheological characterization of the cell-alginate suspensions. Under the investigated conditions, microbeads, 50-600 mu m in diameter, were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with their sizes having higher standard deviations. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5 x 10(8) and 1 x 10(9) cells mL(-1)) did not significantly affect the rheological properties of the Na alginate solution. Finally, we have investigated the kinetics of alginate gelati...on with respect to the quantity of Ca2+ ions and the presence of cells. The molar ratio of alpha-L-guluronic acid units to Ca2+ ions of 4:1 provided complete crosslinking. The presence of cells decreased the rate of network formation as well as the strength of the obtained Ca alginate hydrogel.
Ključne reči:
cell immobilization / rheology / alginate / electrostatic extrusionIzvor:
Journal of Chemical Technology and Biotechnology, 2006, 81, 4, 505-510Izdavač:
- Wiley, Hoboken
DOI: 10.1002/jctb.1465
ISSN: 0268-2575
WoS: 000236946500005
Scopus: 2-s2.0-33646205685
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Manojlović, Verica AU - Đonlagić, Jasna AU - Obradović, Bojana AU - Nedović, Viktor AU - Bugarski, Branko PY - 2006 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/929 AB - In this study, the process of electrostatic extrusion as a method for cell immobilization was investigated. We have assessed the effects of concentrations of yeast cells (as a model cell type) and Na alginate on the size of the resulting microbeads and attempted to rationalize the obtained findings by rheological characterization of the cell-alginate suspensions. Under the investigated conditions, microbeads, 50-600 mu m in diameter, were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with their sizes having higher standard deviations. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5 x 10(8) and 1 x 10(9) cells mL(-1)) did not significantly affect the rheological properties of the Na alginate solution. Finally, we have investigated the kinetics of alginate gelation with respect to the quantity of Ca2+ ions and the presence of cells. The molar ratio of alpha-L-guluronic acid units to Ca2+ ions of 4:1 provided complete crosslinking. The presence of cells decreased the rate of network formation as well as the strength of the obtained Ca alginate hydrogel. PB - Wiley, Hoboken T2 - Journal of Chemical Technology and Biotechnology T1 - Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies EP - 510 IS - 4 SP - 505 VL - 81 DO - 10.1002/jctb.1465 ER -
@article{ author = "Manojlović, Verica and Đonlagić, Jasna and Obradović, Bojana and Nedović, Viktor and Bugarski, Branko", year = "2006", abstract = "In this study, the process of electrostatic extrusion as a method for cell immobilization was investigated. We have assessed the effects of concentrations of yeast cells (as a model cell type) and Na alginate on the size of the resulting microbeads and attempted to rationalize the obtained findings by rheological characterization of the cell-alginate suspensions. Under the investigated conditions, microbeads, 50-600 mu m in diameter, were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with their sizes having higher standard deviations. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5 x 10(8) and 1 x 10(9) cells mL(-1)) did not significantly affect the rheological properties of the Na alginate solution. Finally, we have investigated the kinetics of alginate gelation with respect to the quantity of Ca2+ ions and the presence of cells. The molar ratio of alpha-L-guluronic acid units to Ca2+ ions of 4:1 provided complete crosslinking. The presence of cells decreased the rate of network formation as well as the strength of the obtained Ca alginate hydrogel.", publisher = "Wiley, Hoboken", journal = "Journal of Chemical Technology and Biotechnology", title = "Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies", pages = "510-505", number = "4", volume = "81", doi = "10.1002/jctb.1465" }
Manojlović, V., Đonlagić, J., Obradović, B., Nedović, V.,& Bugarski, B.. (2006). Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies. in Journal of Chemical Technology and Biotechnology Wiley, Hoboken., 81(4), 505-510. https://doi.org/10.1002/jctb.1465
Manojlović V, Đonlagić J, Obradović B, Nedović V, Bugarski B. Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies. in Journal of Chemical Technology and Biotechnology. 2006;81(4):505-510. doi:10.1002/jctb.1465 .
Manojlović, Verica, Đonlagić, Jasna, Obradović, Bojana, Nedović, Viktor, Bugarski, Branko, "Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies" in Journal of Chemical Technology and Biotechnology, 81, no. 4 (2006):505-510, https://doi.org/10.1002/jctb.1465 . .