Kinetics and specificity of Lipozyme-catalysed oil hydrolysis in supercritical CO(2)
Abstract
Blackcurrant seed oil is rich in linoleic and linolenic acids. Selective enzyme-catalysed oil hydrolysis was studied with aim to obtain different levels of alpha- and/or gamma-linolenic acid in the mixture of liberated fatty acids and in the fraction of di- and monoacylglycerols, making them suitable for special dietary needs. The oil was dissolved in supercritical carbon dioxide flowing through a packed bed reactor (temperature 40 degrees C, pressure 15-28 MPa, and superficial velocity 0.1-0.7 mm s(-1)) with Lipozyme(R), a 1,3-specific lipase from Mucor miehei immobilised on a macroporous ion-exchange resin. The enzyme activity was stable as long as water precipitation in the reactor was prevented. The reaction was found to be controlled by both Michaelis-Menten kinetics and mass transfer. The maximum rate of fatty acids liberation per unit amount of enzyme, 2.6 x 10(-3) mol s(-1) kg(-1), was achieved at the maximum flow velocity and pressure. Compared to oil, the liberated fatty acid...s contained more alpha-linolenic, palmitic and stearic acids, while di- and monoacylglycerols contained increased levels of gamma-linolenic and stearidonic acids.
Keywords:
packed bed reactor / enzymatic hydrolysis / supercritical CO(2) / blackcurrant seed oil / 1,3-specific lipaseSource:
Chemical Engineering Research & Design, 2008, 86, 7A, 673-681Publisher:
- Inst Chemical Engineers, Rugby
DOI: 10.1016/j.cherd.2008.03.006
ISSN: 0263-8762
WoS: 000258545800003
Scopus: 2-s2.0-56349102256
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Sovova, H. AU - Zarevucka, M. AU - Bernasek, P. AU - Stamenić, Marko PY - 2008 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1246 AB - Blackcurrant seed oil is rich in linoleic and linolenic acids. Selective enzyme-catalysed oil hydrolysis was studied with aim to obtain different levels of alpha- and/or gamma-linolenic acid in the mixture of liberated fatty acids and in the fraction of di- and monoacylglycerols, making them suitable for special dietary needs. The oil was dissolved in supercritical carbon dioxide flowing through a packed bed reactor (temperature 40 degrees C, pressure 15-28 MPa, and superficial velocity 0.1-0.7 mm s(-1)) with Lipozyme(R), a 1,3-specific lipase from Mucor miehei immobilised on a macroporous ion-exchange resin. The enzyme activity was stable as long as water precipitation in the reactor was prevented. The reaction was found to be controlled by both Michaelis-Menten kinetics and mass transfer. The maximum rate of fatty acids liberation per unit amount of enzyme, 2.6 x 10(-3) mol s(-1) kg(-1), was achieved at the maximum flow velocity and pressure. Compared to oil, the liberated fatty acids contained more alpha-linolenic, palmitic and stearic acids, while di- and monoacylglycerols contained increased levels of gamma-linolenic and stearidonic acids. PB - Inst Chemical Engineers, Rugby T2 - Chemical Engineering Research & Design T1 - Kinetics and specificity of Lipozyme-catalysed oil hydrolysis in supercritical CO(2) EP - 681 IS - 7A SP - 673 VL - 86 DO - 10.1016/j.cherd.2008.03.006 ER -
@article{ author = "Sovova, H. and Zarevucka, M. and Bernasek, P. and Stamenić, Marko", year = "2008", abstract = "Blackcurrant seed oil is rich in linoleic and linolenic acids. Selective enzyme-catalysed oil hydrolysis was studied with aim to obtain different levels of alpha- and/or gamma-linolenic acid in the mixture of liberated fatty acids and in the fraction of di- and monoacylglycerols, making them suitable for special dietary needs. The oil was dissolved in supercritical carbon dioxide flowing through a packed bed reactor (temperature 40 degrees C, pressure 15-28 MPa, and superficial velocity 0.1-0.7 mm s(-1)) with Lipozyme(R), a 1,3-specific lipase from Mucor miehei immobilised on a macroporous ion-exchange resin. The enzyme activity was stable as long as water precipitation in the reactor was prevented. The reaction was found to be controlled by both Michaelis-Menten kinetics and mass transfer. The maximum rate of fatty acids liberation per unit amount of enzyme, 2.6 x 10(-3) mol s(-1) kg(-1), was achieved at the maximum flow velocity and pressure. Compared to oil, the liberated fatty acids contained more alpha-linolenic, palmitic and stearic acids, while di- and monoacylglycerols contained increased levels of gamma-linolenic and stearidonic acids.", publisher = "Inst Chemical Engineers, Rugby", journal = "Chemical Engineering Research & Design", title = "Kinetics and specificity of Lipozyme-catalysed oil hydrolysis in supercritical CO(2)", pages = "681-673", number = "7A", volume = "86", doi = "10.1016/j.cherd.2008.03.006" }
Sovova, H., Zarevucka, M., Bernasek, P.,& Stamenić, M.. (2008). Kinetics and specificity of Lipozyme-catalysed oil hydrolysis in supercritical CO(2). in Chemical Engineering Research & Design Inst Chemical Engineers, Rugby., 86(7A), 673-681. https://doi.org/10.1016/j.cherd.2008.03.006
Sovova H, Zarevucka M, Bernasek P, Stamenić M. Kinetics and specificity of Lipozyme-catalysed oil hydrolysis in supercritical CO(2). in Chemical Engineering Research & Design. 2008;86(7A):673-681. doi:10.1016/j.cherd.2008.03.006 .
Sovova, H., Zarevucka, M., Bernasek, P., Stamenić, Marko, "Kinetics and specificity of Lipozyme-catalysed oil hydrolysis in supercritical CO(2)" in Chemical Engineering Research & Design, 86, no. 7A (2008):673-681, https://doi.org/10.1016/j.cherd.2008.03.006 . .