Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization
Samo za registrovane korisnike
2010
Autori
Milašinović, NikolaMilosavljević, Nedeljko
Filipović, Jovanka
Knežević-Jugović, Zorica
Kalagasidis Krušić, Melina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase.
Ključne reči:
N-Isopropylacrylamide / Itaconic acid / Enzyme immobilization / Candida rugosa lipase / Enzyme activityIzvor:
Reactive & Functional Polymers, 2010, 70, 10, 807-814Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
- Sinteza i karakterizacija polimera i polimernih (nano)kompozita definisane molekulske i nadmolekulske strukture (RS-MESTD-MPN2006-2010-142023)
DOI: 10.1016/j.reactfunctpolym.2010.07.017
ISSN: 1381-5148
WoS: 000282460700014
Scopus: 2-s2.0-77956205453
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Milosavljević, Nedeljko AU - Filipović, Jovanka AU - Knežević-Jugović, Zorica AU - Kalagasidis Krušić, Melina PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1586 AB - Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase. PB - Elsevier Science Bv, Amsterdam T2 - Reactive & Functional Polymers T1 - Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization EP - 814 IS - 10 SP - 807 VL - 70 DO - 10.1016/j.reactfunctpolym.2010.07.017 ER -
@article{ author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina", year = "2010", abstract = "Temperature- and pH-sensitive hydrogels based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA) were applied for immobilization of lipase from Candida rugosa (CRL). The hydrogels were synthesized by free radical crosslinking copolymerization in the presence of lipase. Characterization of samples by swelling studies, at pH 2.20 and 6.80 at a temperature of 37 degrees C, scanning electron microscopy (SEM) and Fourier transform infrared analysis (FT-IR) confirmed that the degree of crosslinking, the nonionic/ionic (NiPAAm/IA) ratio and the enzyme content had impacts on the hydrogel structure, mechanical properties, morphology and swelling kinetics. All hydrogels demonstrated protein loading efficiencies as high as 95 wt.%. A specific activity of the immobilized lipase of around 38 IU/g was attained for an enzyme loading of 20.0 wt.%. As a result, improved pH and temperature optima values were obtained for the immobilized systems in relation to those for the free lipase.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Reactive & Functional Polymers", title = "Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization", pages = "814-807", number = "10", volume = "70", doi = "10.1016/j.reactfunctpolym.2010.07.017" }
Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2010). Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization. in Reactive & Functional Polymers Elsevier Science Bv, Amsterdam., 70(10), 807-814. https://doi.org/10.1016/j.reactfunctpolym.2010.07.017
Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization. in Reactive & Functional Polymers. 2010;70(10):807-814. doi:10.1016/j.reactfunctpolym.2010.07.017 .
Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Synthesis, characterization and application of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels as supports for lipase immobilization" in Reactive & Functional Polymers, 70, no. 10 (2010):807-814, https://doi.org/10.1016/j.reactfunctpolym.2010.07.017 . .