Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein
Само за регистроване кориснике
2010
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Temperature- and pH-sensitive hydrogels, based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), were synthesized by free radical crosslinking copolymerization in the presence of lipase from Candida rugosa. The samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a hydrophilic model protein, lipase. These hydrogels were highly responsive to temperature and pH, at constant ionic strength. Parameters, such as the crosslinking degree and non-ionic/ionic (NiPAAm/IA) ratio, were found to impact the hydrogel structure, mechanical properties, morphology and swelling kinetics at different pH and temperatures. The hydrogels demonstrated protein loading efficiency as high as 95 wt%. Release studies of a hydrophilic model protein at a physiological temperature of 37 degrees C were performed at different pH values. High dependence of lipase release kinetics on hydrogel structure and the environmental pH was found, ...showing generally low release rates, lower in acidic media (pH 2.20) and higher at higher pHs (6.80). Lipase activity was retained even after treatment conditions that would provoke denaturation of the enzyme if it was not protected in the gel. The obtained hydrogels were found suitable for releasing therapeutic proteins in a controlled manner at specific sites in gastrointestinal tract.
Кључне речи:
N-Isopropylacrylamide / Itaconic acid / Environment-sensitive hydrogels / Candida rugosa lipase / Controlled protein release / Release kineticsИзвор:
International Journal of Pharmaceutics, 2010, 383, 1-2, 53-61Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
DOI: 10.1016/j.ijpharm.2009.09.001
ISSN: 0378-5173
PubMed: 19747965
WoS: 000272809800009
Scopus: 2-s2.0-70449553752
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Kalagasidis Krušić, Melina AU - Knežević-Jugović, Zorica AU - Filipović, Jovanka PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1593 AB - Temperature- and pH-sensitive hydrogels, based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), were synthesized by free radical crosslinking copolymerization in the presence of lipase from Candida rugosa. The samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a hydrophilic model protein, lipase. These hydrogels were highly responsive to temperature and pH, at constant ionic strength. Parameters, such as the crosslinking degree and non-ionic/ionic (NiPAAm/IA) ratio, were found to impact the hydrogel structure, mechanical properties, morphology and swelling kinetics at different pH and temperatures. The hydrogels demonstrated protein loading efficiency as high as 95 wt%. Release studies of a hydrophilic model protein at a physiological temperature of 37 degrees C were performed at different pH values. High dependence of lipase release kinetics on hydrogel structure and the environmental pH was found, showing generally low release rates, lower in acidic media (pH 2.20) and higher at higher pHs (6.80). Lipase activity was retained even after treatment conditions that would provoke denaturation of the enzyme if it was not protected in the gel. The obtained hydrogels were found suitable for releasing therapeutic proteins in a controlled manner at specific sites in gastrointestinal tract. PB - Elsevier Science Bv, Amsterdam T2 - International Journal of Pharmaceutics T1 - Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein EP - 61 IS - 1-2 SP - 53 VL - 383 DO - 10.1016/j.ijpharm.2009.09.001 ER -
@article{ author = "Milašinović, Nikola and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica and Filipović, Jovanka", year = "2010", abstract = "Temperature- and pH-sensitive hydrogels, based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), were synthesized by free radical crosslinking copolymerization in the presence of lipase from Candida rugosa. The samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a hydrophilic model protein, lipase. These hydrogels were highly responsive to temperature and pH, at constant ionic strength. Parameters, such as the crosslinking degree and non-ionic/ionic (NiPAAm/IA) ratio, were found to impact the hydrogel structure, mechanical properties, morphology and swelling kinetics at different pH and temperatures. The hydrogels demonstrated protein loading efficiency as high as 95 wt%. Release studies of a hydrophilic model protein at a physiological temperature of 37 degrees C were performed at different pH values. High dependence of lipase release kinetics on hydrogel structure and the environmental pH was found, showing generally low release rates, lower in acidic media (pH 2.20) and higher at higher pHs (6.80). Lipase activity was retained even after treatment conditions that would provoke denaturation of the enzyme if it was not protected in the gel. The obtained hydrogels were found suitable for releasing therapeutic proteins in a controlled manner at specific sites in gastrointestinal tract.", publisher = "Elsevier Science Bv, Amsterdam", journal = "International Journal of Pharmaceutics", title = "Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein", pages = "61-53", number = "1-2", volume = "383", doi = "10.1016/j.ijpharm.2009.09.001" }
Milašinović, N., Kalagasidis Krušić, M., Knežević-Jugović, Z.,& Filipović, J.. (2010). Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein. in International Journal of Pharmaceutics Elsevier Science Bv, Amsterdam., 383(1-2), 53-61. https://doi.org/10.1016/j.ijpharm.2009.09.001
Milašinović N, Kalagasidis Krušić M, Knežević-Jugović Z, Filipović J. Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein. in International Journal of Pharmaceutics. 2010;383(1-2):53-61. doi:10.1016/j.ijpharm.2009.09.001 .
Milašinović, Nikola, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, Filipović, Jovanka, "Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein" in International Journal of Pharmaceutics, 383, no. 1-2 (2010):53-61, https://doi.org/10.1016/j.ijpharm.2009.09.001 . .