Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering
Samo za registrovane korisnike
2010
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial ...interactions under in vivo-like settings.
Izvor:
Journal of Materials Science-Materials in Medicine, 2010, 21, 10, 2869-2879Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Swiss National Science Foundation (SNSF)Swiss National Science Foundation (SNSF) [IB73B0-111016/1]
- Interakcija imobilisanih ćelija, tkiva i biološki aktivnih molekula u bioreaktorskim sistemima (RS-MESTD-MPN2006-2010-142075)
DOI: 10.1007/s10856-010-4135-0
ISSN: 0957-4530
PubMed: 20717710
WoS: 000283371200017
Scopus: 2-s2.0-78349307728
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Stojkovska, Jasmina AU - Bugarski, Branko AU - Obradović, Bojana PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1725 AB - Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings. PB - Springer, Dordrecht T2 - Journal of Materials Science-Materials in Medicine T1 - Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering EP - 2879 IS - 10 SP - 2869 VL - 21 DO - 10.1007/s10856-010-4135-0 ER -
@article{ author = "Stojkovska, Jasmina and Bugarski, Branko and Obradović, Bojana", year = "2010", abstract = "Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.", publisher = "Springer, Dordrecht", journal = "Journal of Materials Science-Materials in Medicine", title = "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering", pages = "2879-2869", number = "10", volume = "21", doi = "10.1007/s10856-010-4135-0" }
Stojkovska, J., Bugarski, B.,& Obradović, B.. (2010). Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. in Journal of Materials Science-Materials in Medicine Springer, Dordrecht., 21(10), 2869-2879. https://doi.org/10.1007/s10856-010-4135-0
Stojkovska J, Bugarski B, Obradović B. Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. in Journal of Materials Science-Materials in Medicine. 2010;21(10):2869-2879. doi:10.1007/s10856-010-4135-0 .
Stojkovska, Jasmina, Bugarski, Branko, Obradović, Bojana, "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering" in Journal of Materials Science-Materials in Medicine, 21, no. 10 (2010):2869-2879, https://doi.org/10.1007/s10856-010-4135-0 . .