Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates
Само за регистроване кориснике
2011
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited highe...r activity at high reaction temperatures and higher thermal stability.
Кључне речи:
Enzyme immobilization / Candida rugosa lipase / Sepabeads (R) / Periodate oxidationИзвор:
Bioprocess and Biosystems Engineering, 2011, 34, 7, 803-810Издавач:
- Springer, New York
Финансирање / пројекти:
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-MESTD-Basic Research (BR or ON)-172013)
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1007/s00449-011-0530-2
ISSN: 1615-7591
PubMed: 21347667
WoS: 000293896200004
Scopus: 2-s2.0-81755187222
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Prlainović, Nevena AU - Knežević-Jugović, Zorica AU - Mijin, Dušan AU - Bezbradica, Dejan PY - 2011 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1939 AB - The objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited higher activity at high reaction temperatures and higher thermal stability. PB - Springer, New York T2 - Bioprocess and Biosystems Engineering T1 - Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates EP - 810 IS - 7 SP - 803 VL - 34 DO - 10.1007/s00449-011-0530-2 ER -
@article{ author = "Prlainović, Nevena and Knežević-Jugović, Zorica and Mijin, Dušan and Bezbradica, Dejan", year = "2011", abstract = "The objective of this paper was the investigation of a suitable Sepabeads(A (R)) support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads(A (R)) EC-EA and Sepabeads(A (R)) EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads(A (R)) EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited higher activity at high reaction temperatures and higher thermal stability.", publisher = "Springer, New York", journal = "Bioprocess and Biosystems Engineering", title = "Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates", pages = "810-803", number = "7", volume = "34", doi = "10.1007/s00449-011-0530-2" }
Prlainović, N., Knežević-Jugović, Z., Mijin, D.,& Bezbradica, D.. (2011). Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates. in Bioprocess and Biosystems Engineering Springer, New York., 34(7), 803-810. https://doi.org/10.1007/s00449-011-0530-2
Prlainović N, Knežević-Jugović Z, Mijin D, Bezbradica D. Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates. in Bioprocess and Biosystems Engineering. 2011;34(7):803-810. doi:10.1007/s00449-011-0530-2 .
Prlainović, Nevena, Knežević-Jugović, Zorica, Mijin, Dušan, Bezbradica, Dejan, "Immobilization of lipase from Candida rugosa on Sepabeads(A (R)): the effect of lipase oxidation by periodates" in Bioprocess and Biosystems Engineering, 34, no. 7 (2011):803-810, https://doi.org/10.1007/s00449-011-0530-2 . .