Comparative Study of Batch and Fluidized Bed Bioreactors for Lipase-Catalyzed Ethyl Cinnamate Synthesis
Samo za registrovane korisnike
2013
Autori
Jakovetić, SonjaLuković, Nevena
Bošković-Vragolović, Nevenka
Bezbradica, Dejan
Picazo-Espinosa, Rafael
Knežević-Jugović, Zorica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Ethyl cinnamate, an ester known as flavor and fragrance compound, has been synthesized using two immobilized bioreactor systems, batch and fluidized bed bioreactors. The enzyme used for this synthesis is a commercial lipase B preparation, Novozyme 435. Initial kinetic studies were conducted in both employed bioreactor configurations, and kinetic constants were obtained. Several models were tried for fitting of experimental data, but the best fit, for both bioreactors, was obtained when the ping-pong bi-bi mechanism was used. Interestingly enough, ethanol inhibition occurred in batch bioreactor, but it did not exist in the fluidized bed bioreactor. Solid-liquid mass transfer coefficients were calculated for both bioreactors to determine whether mass transfer limitations existed in either of these systems. The calculation of Damkohler numbers and Thiele modulus confirmed that mass transfer limitations had no effect on the overall reaction in both bioreactors.
Izvor:
Industrial & Engineering Chemistry Research, 2013, 52, 47, 16689-16697Izdavač:
- Amer Chemical Soc, Washington
Finansiranje / projekti:
- E!6750
DOI: 10.1021/ie402069c
ISSN: 0888-5885
WoS: 000327812400016
Scopus: 2-s2.0-84889243686
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Jakovetić, Sonja AU - Luković, Nevena AU - Bošković-Vragolović, Nevenka AU - Bezbradica, Dejan AU - Picazo-Espinosa, Rafael AU - Knežević-Jugović, Zorica PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2431 AB - Ethyl cinnamate, an ester known as flavor and fragrance compound, has been synthesized using two immobilized bioreactor systems, batch and fluidized bed bioreactors. The enzyme used for this synthesis is a commercial lipase B preparation, Novozyme 435. Initial kinetic studies were conducted in both employed bioreactor configurations, and kinetic constants were obtained. Several models were tried for fitting of experimental data, but the best fit, for both bioreactors, was obtained when the ping-pong bi-bi mechanism was used. Interestingly enough, ethanol inhibition occurred in batch bioreactor, but it did not exist in the fluidized bed bioreactor. Solid-liquid mass transfer coefficients were calculated for both bioreactors to determine whether mass transfer limitations existed in either of these systems. The calculation of Damkohler numbers and Thiele modulus confirmed that mass transfer limitations had no effect on the overall reaction in both bioreactors. PB - Amer Chemical Soc, Washington T2 - Industrial & Engineering Chemistry Research T1 - Comparative Study of Batch and Fluidized Bed Bioreactors for Lipase-Catalyzed Ethyl Cinnamate Synthesis EP - 16697 IS - 47 SP - 16689 VL - 52 DO - 10.1021/ie402069c ER -
@article{ author = "Jakovetić, Sonja and Luković, Nevena and Bošković-Vragolović, Nevenka and Bezbradica, Dejan and Picazo-Espinosa, Rafael and Knežević-Jugović, Zorica", year = "2013", abstract = "Ethyl cinnamate, an ester known as flavor and fragrance compound, has been synthesized using two immobilized bioreactor systems, batch and fluidized bed bioreactors. The enzyme used for this synthesis is a commercial lipase B preparation, Novozyme 435. Initial kinetic studies were conducted in both employed bioreactor configurations, and kinetic constants were obtained. Several models were tried for fitting of experimental data, but the best fit, for both bioreactors, was obtained when the ping-pong bi-bi mechanism was used. Interestingly enough, ethanol inhibition occurred in batch bioreactor, but it did not exist in the fluidized bed bioreactor. Solid-liquid mass transfer coefficients were calculated for both bioreactors to determine whether mass transfer limitations existed in either of these systems. The calculation of Damkohler numbers and Thiele modulus confirmed that mass transfer limitations had no effect on the overall reaction in both bioreactors.", publisher = "Amer Chemical Soc, Washington", journal = "Industrial & Engineering Chemistry Research", title = "Comparative Study of Batch and Fluidized Bed Bioreactors for Lipase-Catalyzed Ethyl Cinnamate Synthesis", pages = "16697-16689", number = "47", volume = "52", doi = "10.1021/ie402069c" }
Jakovetić, S., Luković, N., Bošković-Vragolović, N., Bezbradica, D., Picazo-Espinosa, R.,& Knežević-Jugović, Z.. (2013). Comparative Study of Batch and Fluidized Bed Bioreactors for Lipase-Catalyzed Ethyl Cinnamate Synthesis. in Industrial & Engineering Chemistry Research Amer Chemical Soc, Washington., 52(47), 16689-16697. https://doi.org/10.1021/ie402069c
Jakovetić S, Luković N, Bošković-Vragolović N, Bezbradica D, Picazo-Espinosa R, Knežević-Jugović Z. Comparative Study of Batch and Fluidized Bed Bioreactors for Lipase-Catalyzed Ethyl Cinnamate Synthesis. in Industrial & Engineering Chemistry Research. 2013;52(47):16689-16697. doi:10.1021/ie402069c .
Jakovetić, Sonja, Luković, Nevena, Bošković-Vragolović, Nevenka, Bezbradica, Dejan, Picazo-Espinosa, Rafael, Knežević-Jugović, Zorica, "Comparative Study of Batch and Fluidized Bed Bioreactors for Lipase-Catalyzed Ethyl Cinnamate Synthesis" in Industrial & Engineering Chemistry Research, 52, no. 47 (2013):16689-16697, https://doi.org/10.1021/ie402069c . .