Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
Samo za registrovane korisnike
2013
Autori
Prlainović, NevenaBezbradica, Dejan
Knežević-Jugović, Zorica
Stevanović, Sanja
Avramov-Ivić, Milka
Uskoković, Petar
Mijin, Dušan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
Ključne reči:
Lipase / Carbon nanotubes / Adsorption / FT-IR spectroscopy / AFM / Cyclic voltammetryIzvor:
Journal of Industrial and Engineering Chemistry, 2013, 19, 1, 279-285Izdavač:
- Elsevier Science Inc, New York
Finansiranje / projekti:
- Proučavanje sinteze, strukture i aktivnosti organskih jedinjenja prirodnog i sintetskog porekla (RS-MESTD-Basic Research (BR or ON)-172013)
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Sinteza, razvoj tehnologija dobijanja i primena nanostrukturnih multifunkcionalnih materijala definisanih svojstava (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45019)
DOI: 10.1016/j.jiec.2012.08.012
ISSN: 1226-086X
WoS: 000312764700039
Scopus: 2-s2.0-84870399209
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Prlainović, Nevena AU - Bezbradica, Dejan AU - Knežević-Jugović, Zorica AU - Stevanović, Sanja AU - Avramov-Ivić, Milka AU - Uskoković, Petar AU - Mijin, Dušan PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2477 AB - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry. PB - Elsevier Science Inc, New York T2 - Journal of Industrial and Engineering Chemistry T1 - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes EP - 285 IS - 1 SP - 279 VL - 19 DO - 10.1016/j.jiec.2012.08.012 ER -
@article{ author = "Prlainović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov-Ivić, Milka and Uskoković, Petar and Mijin, Dušan", year = "2013", abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.", publisher = "Elsevier Science Inc, New York", journal = "Journal of Industrial and Engineering Chemistry", title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes", pages = "285-279", number = "1", volume = "19", doi = "10.1016/j.jiec.2012.08.012" }
Prlainović, N., Bezbradica, D., Knežević-Jugović, Z., Stevanović, S., Avramov-Ivić, M., Uskoković, P.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry Elsevier Science Inc, New York., 19(1), 279-285. https://doi.org/10.1016/j.jiec.2012.08.012
Prlainović N, Bezbradica D, Knežević-Jugović Z, Stevanović S, Avramov-Ivić M, Uskoković P, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285. doi:10.1016/j.jiec.2012.08.012 .
Prlainović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov-Ivić, Milka, Uskoković, Petar, Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285, https://doi.org/10.1016/j.jiec.2012.08.012 . .