Kinetic model of lipase-catalyzed conversion of ascorbic acid and oleic acid to liposoluble vitamin C ester
Samo za registrovane korisnike
2013
Autori
Bezbradica, DejanStojanović, Marija
Veličković, Dušan
Dimitrijević, Aleksandra
Carević, Milica
Mihailović, Mladen
Milosavić, Nenad
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The kinetics of L-ascorbyl oleate synthesis catalyzed by immobilized lipase from Candida antarctica in acetone was investigated. Significant inhibition of synthesis with an excess of ascorbic acid was observed. Experimental data were successfully fitted with a ping-pong bi-bi kinetic model with substrate inhibition, and related kinetic constants were determined. The kinetic study was performed at optimum experimental factors (temperature, initial water content, and enzyme concentration), which were determined using response surface methodology. Then, a model for predicting product-time progress curves was developed by expanding the obtained ping-pang model with terms describing ester hydrolysis. Kinetic constants of the reverse reaction were determined, and good congruence between the model and experimental data was achieved. Calculated kinetic constants revealed that lipase has the highest affinity for ascorbyl oleate, slightly lower activity with ascorbic acid, and the lowest activit...y with oleic acid. The obtained results are valuable for elucidating the reaction mechanism and represent an important contribution for reaction optimization and creating strategies to increase the productivity of vitamin C ester synthesis.
Ključne reči:
Ascorbyl oleate / Lipase / Candida antarctica / Kinetic parameters / Substrate inhibition / Production kineticsIzvor:
Biochemical Engineering Journal, 2013, 71, 89-96Izdavač:
- Elsevier Science Sa, Lausanne
Finansiranje / projekti:
DOI: 10.1016/j.bej.2012.12.001
ISSN: 1369-703X
WoS: 000315016800012
Scopus: 2-s2.0-84872407885
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Bezbradica, Dejan AU - Stojanović, Marija AU - Veličković, Dušan AU - Dimitrijević, Aleksandra AU - Carević, Milica AU - Mihailović, Mladen AU - Milosavić, Nenad PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2550 AB - The kinetics of L-ascorbyl oleate synthesis catalyzed by immobilized lipase from Candida antarctica in acetone was investigated. Significant inhibition of synthesis with an excess of ascorbic acid was observed. Experimental data were successfully fitted with a ping-pong bi-bi kinetic model with substrate inhibition, and related kinetic constants were determined. The kinetic study was performed at optimum experimental factors (temperature, initial water content, and enzyme concentration), which were determined using response surface methodology. Then, a model for predicting product-time progress curves was developed by expanding the obtained ping-pang model with terms describing ester hydrolysis. Kinetic constants of the reverse reaction were determined, and good congruence between the model and experimental data was achieved. Calculated kinetic constants revealed that lipase has the highest affinity for ascorbyl oleate, slightly lower activity with ascorbic acid, and the lowest activity with oleic acid. The obtained results are valuable for elucidating the reaction mechanism and represent an important contribution for reaction optimization and creating strategies to increase the productivity of vitamin C ester synthesis. PB - Elsevier Science Sa, Lausanne T2 - Biochemical Engineering Journal T1 - Kinetic model of lipase-catalyzed conversion of ascorbic acid and oleic acid to liposoluble vitamin C ester EP - 96 SP - 89 VL - 71 DO - 10.1016/j.bej.2012.12.001 ER -
@article{ author = "Bezbradica, Dejan and Stojanović, Marija and Veličković, Dušan and Dimitrijević, Aleksandra and Carević, Milica and Mihailović, Mladen and Milosavić, Nenad", year = "2013", abstract = "The kinetics of L-ascorbyl oleate synthesis catalyzed by immobilized lipase from Candida antarctica in acetone was investigated. Significant inhibition of synthesis with an excess of ascorbic acid was observed. Experimental data were successfully fitted with a ping-pong bi-bi kinetic model with substrate inhibition, and related kinetic constants were determined. The kinetic study was performed at optimum experimental factors (temperature, initial water content, and enzyme concentration), which were determined using response surface methodology. Then, a model for predicting product-time progress curves was developed by expanding the obtained ping-pang model with terms describing ester hydrolysis. Kinetic constants of the reverse reaction were determined, and good congruence between the model and experimental data was achieved. Calculated kinetic constants revealed that lipase has the highest affinity for ascorbyl oleate, slightly lower activity with ascorbic acid, and the lowest activity with oleic acid. The obtained results are valuable for elucidating the reaction mechanism and represent an important contribution for reaction optimization and creating strategies to increase the productivity of vitamin C ester synthesis.", publisher = "Elsevier Science Sa, Lausanne", journal = "Biochemical Engineering Journal", title = "Kinetic model of lipase-catalyzed conversion of ascorbic acid and oleic acid to liposoluble vitamin C ester", pages = "96-89", volume = "71", doi = "10.1016/j.bej.2012.12.001" }
Bezbradica, D., Stojanović, M., Veličković, D., Dimitrijević, A., Carević, M., Mihailović, M.,& Milosavić, N.. (2013). Kinetic model of lipase-catalyzed conversion of ascorbic acid and oleic acid to liposoluble vitamin C ester. in Biochemical Engineering Journal Elsevier Science Sa, Lausanne., 71, 89-96. https://doi.org/10.1016/j.bej.2012.12.001
Bezbradica D, Stojanović M, Veličković D, Dimitrijević A, Carević M, Mihailović M, Milosavić N. Kinetic model of lipase-catalyzed conversion of ascorbic acid and oleic acid to liposoluble vitamin C ester. in Biochemical Engineering Journal. 2013;71:89-96. doi:10.1016/j.bej.2012.12.001 .
Bezbradica, Dejan, Stojanović, Marija, Veličković, Dušan, Dimitrijević, Aleksandra, Carević, Milica, Mihailović, Mladen, Milosavić, Nenad, "Kinetic model of lipase-catalyzed conversion of ascorbic acid and oleic acid to liposoluble vitamin C ester" in Biochemical Engineering Journal, 71 (2013):89-96, https://doi.org/10.1016/j.bej.2012.12.001 . .