Приказ основних података о документу
Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure
dc.creator | Ružić, Đurđina | |
dc.creator | Vujović, Tatjana | |
dc.creator | Cerović, Radosav | |
dc.date.accessioned | 2021-03-10T12:17:52Z | |
dc.date.available | 2021-03-10T12:17:52Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0862-867X | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2560 | |
dc.description.abstract | In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype. | en |
dc.publisher | Czech Academy of Agricultural Sciences | |
dc.rights | openAccess | |
dc.source | Horticultural Science | |
dc.subject | Prunus cerasus x Prunus canescens | en |
dc.subject | liquid nitrogen | en |
dc.subject | vitrification solutions | en |
dc.title | Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 63 | |
dc.citation.issue | 2 | |
dc.citation.other | 41(2): 55-63 | |
dc.citation.rank | M23 | |
dc.citation.spage | 55 | |
dc.citation.volume | 41 | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/11788/234_2013-HORTSCI.pdf | |
dc.identifier.pmid | ||
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_technorep_2560 | |
dc.identifier.scopus | 2-s2.0-84902440320 | |
dc.identifier.wos | 000338096300002 | |
dc.type.version | publishedVersion |