Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
2014
Autori
Milašinović, NikolaJakovetić, Sonja
Knežević-Jugović, Zorica
Milosavljević, Nedeljko
Lučić, Marija
Filipović, Jovanka
Kalagasidis Krušić, Melina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipa...se was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
Izvor:
Scientific World Journal, 2014Izdavač:
- Hindawi Publishing Corp, New York
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-46010)
- Sinteza i karakterizacija novih funkcionalnih polimera i polimernih nanokompozita (RS-172062)
DOI: 10.1155/2014/142123
ISSN: 1537-744X
PubMed: 24701136
WoS: 000332230600001
Scopus: 2-s2.0-84896343481
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Jakovetić, Sonja AU - Knežević-Jugović, Zorica AU - Milosavljević, Nedeljko AU - Lučić, Marija AU - Filipović, Jovanka AU - Kalagasidis Krušić, Melina PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2771 AB - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale. PB - Hindawi Publishing Corp, New York T2 - Scientific World Journal T1 - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel DO - 10.1155/2014/142123 ER -
@article{ author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lučić, Marija and Filipović, Jovanka and Kalagasidis Krušić, Melina", year = "2014", abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.", publisher = "Hindawi Publishing Corp, New York", journal = "Scientific World Journal", title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel", doi = "10.1155/2014/142123" }
Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lučić, M., Filipović, J.,& Kalagasidis Krušić, M.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal Hindawi Publishing Corp, New York.. https://doi.org/10.1155/2014/142123
Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lučić M, Filipović J, Kalagasidis Krušić M. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal. 2014;. doi:10.1155/2014/142123 .
Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific World Journal (2014), https://doi.org/10.1155/2014/142123 . .