dc.creator | Radovanović, Željko | |
dc.creator | Jokić, Bojan | |
dc.creator | Veljović, Đorđe | |
dc.creator | Dimitrijević, Suzana | |
dc.creator | Kojić, Vesna | |
dc.creator | Petrović, Rada | |
dc.creator | Janaćković, Đorđe | |
dc.date.accessioned | 2021-03-10T12:34:14Z | |
dc.date.available | 2021-03-10T12:34:14Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0169-4332 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2821 | |
dc.description.abstract | Hydroxyapatite (HAp) powders doped with Ag+ or Cu2+ were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (Ag+ or Cu2+) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200 C for 2 h with the intention of investigating the influence of doping with Ag+ and Cu2+ on the creation of the biphasic HAp/alpha-tricalcium phosphate (HAp/a-TCP) and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/a-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and energydispersive X-ray spectroscopy (EDS). The in vitro antibacterial activities of the powders were evaluated against: Staphylococcus aureus, Escherichia co li, Pseudomonas aeruginosa and Candida albi cans. All powders showed good antimicrobial activity but generally the powders of doped HAp/a-TCP had more uniform results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MU and DET, were used to evaluate the biocompatibility of Ag+- and Cu2+-doped HAp/a-TCP with MRC-5 human fibroblast cells. These tests confirmed that powders do not have a cytotoxic effect. The HAp/a-TCP powders doped with the lower content of Ag+ and Cu2+ showed especially good biocompatibility. Antimicrobial and biocompatibility tests recommend the Ag+- and Cu2+-doped HAp/a-TCP as promising material for use in reconstructive surgery of bone. | en |
dc.publisher | Elsevier Science Bv, Amsterdam | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/245916/EU// | |
dc.rights | restrictedAccess | |
dc.source | Applied Surface Science | |
dc.subject | HAp/alpha-TCP | en |
dc.subject | Ag+-doped | en |
dc.subject | Cu2+-doped | en |
dc.subject | Antimicrobial | en |
dc.subject | Biocompatibility | en |
dc.title | Antimicrobial activity and biocompatibility of Ag--(+) and Cu2+ -doped biphasic hydroxyapatite/alpha-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+ -doped hydroxyapatite | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 519 | |
dc.citation.other | 307: 513-519 | |
dc.citation.rank | M21 | |
dc.citation.spage | 513 | |
dc.citation.volume | 307 | |
dc.identifier.doi | 10.1016/j.apsusc.2014.04.066 | |
dc.identifier.scopus | 2-s2.0-84901326629 | |
dc.identifier.wos | 000336596700071 | |
dc.type.version | publishedVersion | |