ZnO-modified cellulose fiber sheets for antibody immobilization
Samo za registrovane korisnike
2014
Autori
Khatri, VinayHalasz, Katalin
Trandafilović, Lidija V.
Dimitrijević-Branković, Suzana
Mohanty, Paritosh
Đoković, Vladimir
Csoka, Levente
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Cellulose fiber sheets impregnated with saccharide capped-ZnO nanoparticles were used as bioactive materials for antibody immobilization. First, ZnO nanoparticles were synthesized in the presence of glucose (monosaccharide), sucrose (disaccharide) as well as alginic acid and starch (polysaccharides). The pine cellulose fibers were then modified by the obtained saccharide capped nanoparticles and further incorporated into the sheets. The presence of ZnO significantly improved the immobilization of the antibodies on the surface of the sheets. After rewetting the alginic acid-ZnO modified sheets with saline solution, the retention of antibodies was about 95%. A high degree of the immobilization of biomolecules is an important feature for possible fabrications of bioactive- or biosensing-papers and we successfully tested the sheets on the detection of blood types using (A, B, and D blood antibodies). The ZnO nanopartides affected also the other properties of the sheets. The ZnO-modified fi...ber sheets showed higher values of tensile index (strength), smoothness and opacity, while the value of porosity was substantially lower than that of the unmodified sheet. The presence of ZnO nanoparticles provided also the antimicrobial activity to the sheets. They showed a strong activity against bacteria (Escherichia coli and Staphylococcus aureus) and strong resistance to the attack of cellulase producing fungus Gloeophyllum trabeum.
Ključne reči:
Antibody immobilization / Cellulose / Fibers / ZnO / Antimicrobial properties / Bioactive paperIzvor:
Carbohydrate Polymers, 2014, 109, 139-147Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Environment conscious energy efficient building project - EU [TAMOP-4.2.2.A-11/1/KONV-2012-0068]
- European Social Foundation
- Uticaj veličine, oblika i strukture nanočestica na njihova svojstva i svojstva nanokompozita (RS-MESTD-Basic Research (BR or ON)-172056)
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
DOI: 10.1016/j.carbpol.2014.03.061
ISSN: 0144-8617
PubMed: 24815410
WoS: 000336711500018
Scopus: 2-s2.0-84925835395
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Khatri, Vinay AU - Halasz, Katalin AU - Trandafilović, Lidija V. AU - Dimitrijević-Branković, Suzana AU - Mohanty, Paritosh AU - Đoković, Vladimir AU - Csoka, Levente PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2823 AB - Cellulose fiber sheets impregnated with saccharide capped-ZnO nanoparticles were used as bioactive materials for antibody immobilization. First, ZnO nanoparticles were synthesized in the presence of glucose (monosaccharide), sucrose (disaccharide) as well as alginic acid and starch (polysaccharides). The pine cellulose fibers were then modified by the obtained saccharide capped nanoparticles and further incorporated into the sheets. The presence of ZnO significantly improved the immobilization of the antibodies on the surface of the sheets. After rewetting the alginic acid-ZnO modified sheets with saline solution, the retention of antibodies was about 95%. A high degree of the immobilization of biomolecules is an important feature for possible fabrications of bioactive- or biosensing-papers and we successfully tested the sheets on the detection of blood types using (A, B, and D blood antibodies). The ZnO nanopartides affected also the other properties of the sheets. The ZnO-modified fiber sheets showed higher values of tensile index (strength), smoothness and opacity, while the value of porosity was substantially lower than that of the unmodified sheet. The presence of ZnO nanoparticles provided also the antimicrobial activity to the sheets. They showed a strong activity against bacteria (Escherichia coli and Staphylococcus aureus) and strong resistance to the attack of cellulase producing fungus Gloeophyllum trabeum. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Polymers T1 - ZnO-modified cellulose fiber sheets for antibody immobilization EP - 147 SP - 139 VL - 109 DO - 10.1016/j.carbpol.2014.03.061 ER -
@article{ author = "Khatri, Vinay and Halasz, Katalin and Trandafilović, Lidija V. and Dimitrijević-Branković, Suzana and Mohanty, Paritosh and Đoković, Vladimir and Csoka, Levente", year = "2014", abstract = "Cellulose fiber sheets impregnated with saccharide capped-ZnO nanoparticles were used as bioactive materials for antibody immobilization. First, ZnO nanoparticles were synthesized in the presence of glucose (monosaccharide), sucrose (disaccharide) as well as alginic acid and starch (polysaccharides). The pine cellulose fibers were then modified by the obtained saccharide capped nanoparticles and further incorporated into the sheets. The presence of ZnO significantly improved the immobilization of the antibodies on the surface of the sheets. After rewetting the alginic acid-ZnO modified sheets with saline solution, the retention of antibodies was about 95%. A high degree of the immobilization of biomolecules is an important feature for possible fabrications of bioactive- or biosensing-papers and we successfully tested the sheets on the detection of blood types using (A, B, and D blood antibodies). The ZnO nanopartides affected also the other properties of the sheets. The ZnO-modified fiber sheets showed higher values of tensile index (strength), smoothness and opacity, while the value of porosity was substantially lower than that of the unmodified sheet. The presence of ZnO nanoparticles provided also the antimicrobial activity to the sheets. They showed a strong activity against bacteria (Escherichia coli and Staphylococcus aureus) and strong resistance to the attack of cellulase producing fungus Gloeophyllum trabeum.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Polymers", title = "ZnO-modified cellulose fiber sheets for antibody immobilization", pages = "147-139", volume = "109", doi = "10.1016/j.carbpol.2014.03.061" }
Khatri, V., Halasz, K., Trandafilović, L. V., Dimitrijević-Branković, S., Mohanty, P., Đoković, V.,& Csoka, L.. (2014). ZnO-modified cellulose fiber sheets for antibody immobilization. in Carbohydrate Polymers Elsevier Sci Ltd, Oxford., 109, 139-147. https://doi.org/10.1016/j.carbpol.2014.03.061
Khatri V, Halasz K, Trandafilović LV, Dimitrijević-Branković S, Mohanty P, Đoković V, Csoka L. ZnO-modified cellulose fiber sheets for antibody immobilization. in Carbohydrate Polymers. 2014;109:139-147. doi:10.1016/j.carbpol.2014.03.061 .
Khatri, Vinay, Halasz, Katalin, Trandafilović, Lidija V., Dimitrijević-Branković, Suzana, Mohanty, Paritosh, Đoković, Vladimir, Csoka, Levente, "ZnO-modified cellulose fiber sheets for antibody immobilization" in Carbohydrate Polymers, 109 (2014):139-147, https://doi.org/10.1016/j.carbpol.2014.03.061 . .