Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae
Само за регистроване кориснике
2014
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
2-Ketogluconic acid production by Klebsiella pneumoniae is a pH-dependent process, strictly proceeding under acidic conditions. Unfortunately, cell growth is inhibited by acidic conditions, resulting in low productivity of 2-ketogluconic acid. To overcome this deficiency, a two-stage fermentation strategy was exploited in the current study. During the first stage, the culture was maintained at neutral pH, favoring cell growth. During the second stage, the culture pH was switched to acidic conditions favoring 2-ketogluconic acid accumulation. Culture parameters, including switching time, dissolved oxygen levels, pH, and temperature were optimized for the fed-batch fermentation. Characteristics of glucose dehydrogenase and gluconate dehydrogenase were revealed in vitro, and the optimal pHs of the two enzymes coincided with the optimum culture pH. Under optimum conditions, a total of 186 g/l 2-ketogluconic acid was produced at 26 h, and the conversion ratio was 0.98 mol/mol. This fermenta...tion strategy has successfully overcome the mismatch between optimum parameters required for cell growth and 2-ketogluconic acid accumulation, and this result has the highest productivity and conversion ratio of 2-ketogluconic and produced by microorganism.
Кључне речи:
2-Ketogluconic acid / Klebsiella pneumoniae / two-stage fermentation / erythorbic acidИзвор:
Journal of Microbiology and Biotechnology, 2014, 24, 6, 781-787Издавач:
- Korean Soc Microbiology & Biotechnology, Seoul
Финансирање / пројекти:
- National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [20906076]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education MinistryScientific Research Foundation for the Returned Overseas Chinese Scholars
- Serbian-Chinese Science & Technology Cooperation [1-3]
DOI: 10.4014/jmb.1401.01038
ISSN: 1017-7825
PubMed: 24572278
WoS: 000338177600008
Scopus: 2-s2.0-84902595551
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Sun, Yuehong AU - Wei, Dong AU - Shi, Jiping AU - Mojović, Ljiljana AU - Han, Zengsheng AU - Hao, Jian PY - 2014 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2832 AB - 2-Ketogluconic acid production by Klebsiella pneumoniae is a pH-dependent process, strictly proceeding under acidic conditions. Unfortunately, cell growth is inhibited by acidic conditions, resulting in low productivity of 2-ketogluconic acid. To overcome this deficiency, a two-stage fermentation strategy was exploited in the current study. During the first stage, the culture was maintained at neutral pH, favoring cell growth. During the second stage, the culture pH was switched to acidic conditions favoring 2-ketogluconic acid accumulation. Culture parameters, including switching time, dissolved oxygen levels, pH, and temperature were optimized for the fed-batch fermentation. Characteristics of glucose dehydrogenase and gluconate dehydrogenase were revealed in vitro, and the optimal pHs of the two enzymes coincided with the optimum culture pH. Under optimum conditions, a total of 186 g/l 2-ketogluconic acid was produced at 26 h, and the conversion ratio was 0.98 mol/mol. This fermentation strategy has successfully overcome the mismatch between optimum parameters required for cell growth and 2-ketogluconic acid accumulation, and this result has the highest productivity and conversion ratio of 2-ketogluconic and produced by microorganism. PB - Korean Soc Microbiology & Biotechnology, Seoul T2 - Journal of Microbiology and Biotechnology T1 - Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae EP - 787 IS - 6 SP - 781 VL - 24 DO - 10.4014/jmb.1401.01038 ER -
@article{ author = "Sun, Yuehong and Wei, Dong and Shi, Jiping and Mojović, Ljiljana and Han, Zengsheng and Hao, Jian", year = "2014", abstract = "2-Ketogluconic acid production by Klebsiella pneumoniae is a pH-dependent process, strictly proceeding under acidic conditions. Unfortunately, cell growth is inhibited by acidic conditions, resulting in low productivity of 2-ketogluconic acid. To overcome this deficiency, a two-stage fermentation strategy was exploited in the current study. During the first stage, the culture was maintained at neutral pH, favoring cell growth. During the second stage, the culture pH was switched to acidic conditions favoring 2-ketogluconic acid accumulation. Culture parameters, including switching time, dissolved oxygen levels, pH, and temperature were optimized for the fed-batch fermentation. Characteristics of glucose dehydrogenase and gluconate dehydrogenase were revealed in vitro, and the optimal pHs of the two enzymes coincided with the optimum culture pH. Under optimum conditions, a total of 186 g/l 2-ketogluconic acid was produced at 26 h, and the conversion ratio was 0.98 mol/mol. This fermentation strategy has successfully overcome the mismatch between optimum parameters required for cell growth and 2-ketogluconic acid accumulation, and this result has the highest productivity and conversion ratio of 2-ketogluconic and produced by microorganism.", publisher = "Korean Soc Microbiology & Biotechnology, Seoul", journal = "Journal of Microbiology and Biotechnology", title = "Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae", pages = "787-781", number = "6", volume = "24", doi = "10.4014/jmb.1401.01038" }
Sun, Y., Wei, D., Shi, J., Mojović, L., Han, Z.,& Hao, J.. (2014). Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae. in Journal of Microbiology and Biotechnology Korean Soc Microbiology & Biotechnology, Seoul., 24(6), 781-787. https://doi.org/10.4014/jmb.1401.01038
Sun Y, Wei D, Shi J, Mojović L, Han Z, Hao J. Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae. in Journal of Microbiology and Biotechnology. 2014;24(6):781-787. doi:10.4014/jmb.1401.01038 .
Sun, Yuehong, Wei, Dong, Shi, Jiping, Mojović, Ljiljana, Han, Zengsheng, Hao, Jian, "Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae" in Journal of Microbiology and Biotechnology, 24, no. 6 (2014):781-787, https://doi.org/10.4014/jmb.1401.01038 . .