Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain
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2015
Authors
Grbavčić, SanjaMarković, Darka
Rajilić-Stojanović, Mirjana
Antov, Mirjana
Sciban, Marina
Karadžić, Ivanka
Knežević-Jugović, Zorica
Article (Published version)
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A lipase derived from an indigenous extremophile Pseudomonas aeruginosa strain isolated from rancid metalworking fluid was evaluated as a detergent additive. Applicability of the obtained enzyme as an additive in detergent formulations was confirmed by its implementation in the formulations of several new products differing in surfactant type and concentrations, demonstrating satisfactory performance in terms of degreasing efficiency and composition of the washing wastewater. The degreasing efficiency of different enzyme-containing detergent formulations was studied on cotton fabric samples stained with triolein and compared to that of formulations containing only surfactant. The highest efficiency of the fatty soil removal in formulations with a low content of surfactants (0.4 %) was noted in the enzyme formulation containing Lutensol(A (R)) XP-80 (degreasing efficiency gt 80 %) and Triton(A (R)) X-100 (degreasing efficiency gt 60 %). An attempt was then made to optimize the compo...sition of the enzyme formulation on the basis of one or both of these surfactants using statistically planned experiments and response surface methodology (RSM). Taking into consideration the environmental aspects and the shown detergency, it appeared that rather high degreasing effects were achieved in formulations based on a low quantities of Lutensol(A (R)) XP-80 (0.4 %) at all pH values. However, pH seemed to have a notable effect since the degreasing efficiency significantly increased with increasing pH and the amount of the enzyme. Formulations having a moderate alkaline pH profile and higher amount of enzyme exhibited a high cleaning performance of fatty soil even at a low concentration of the surfactant.
Keywords:
Lipase / Pseudomonas aeruginosa / Detergent / Surfactant / Environmentally friendly formulationSource:
Journal of Surfactants and Detergents, 2015, 18, 3, 383-395Publisher:
- Wiley, Hoboken
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
DOI: 10.1007/s11743-015-1674-x
ISSN: 1097-3958
WoS: 000351665600002
Scopus: 2-s2.0-84939938464
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Tehnološko-metalurški fakultetTY - JOUR AU - Grbavčić, Sanja AU - Marković, Darka AU - Rajilić-Stojanović, Mirjana AU - Antov, Mirjana AU - Sciban, Marina AU - Karadžić, Ivanka AU - Knežević-Jugović, Zorica PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2977 AB - A lipase derived from an indigenous extremophile Pseudomonas aeruginosa strain isolated from rancid metalworking fluid was evaluated as a detergent additive. Applicability of the obtained enzyme as an additive in detergent formulations was confirmed by its implementation in the formulations of several new products differing in surfactant type and concentrations, demonstrating satisfactory performance in terms of degreasing efficiency and composition of the washing wastewater. The degreasing efficiency of different enzyme-containing detergent formulations was studied on cotton fabric samples stained with triolein and compared to that of formulations containing only surfactant. The highest efficiency of the fatty soil removal in formulations with a low content of surfactants (0.4 %) was noted in the enzyme formulation containing Lutensol(A (R)) XP-80 (degreasing efficiency gt 80 %) and Triton(A (R)) X-100 (degreasing efficiency gt 60 %). An attempt was then made to optimize the composition of the enzyme formulation on the basis of one or both of these surfactants using statistically planned experiments and response surface methodology (RSM). Taking into consideration the environmental aspects and the shown detergency, it appeared that rather high degreasing effects were achieved in formulations based on a low quantities of Lutensol(A (R)) XP-80 (0.4 %) at all pH values. However, pH seemed to have a notable effect since the degreasing efficiency significantly increased with increasing pH and the amount of the enzyme. Formulations having a moderate alkaline pH profile and higher amount of enzyme exhibited a high cleaning performance of fatty soil even at a low concentration of the surfactant. PB - Wiley, Hoboken T2 - Journal of Surfactants and Detergents T1 - Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain EP - 395 IS - 3 SP - 383 VL - 18 DO - 10.1007/s11743-015-1674-x ER -
@article{ author = "Grbavčić, Sanja and Marković, Darka and Rajilić-Stojanović, Mirjana and Antov, Mirjana and Sciban, Marina and Karadžić, Ivanka and Knežević-Jugović, Zorica", year = "2015", abstract = "A lipase derived from an indigenous extremophile Pseudomonas aeruginosa strain isolated from rancid metalworking fluid was evaluated as a detergent additive. Applicability of the obtained enzyme as an additive in detergent formulations was confirmed by its implementation in the formulations of several new products differing in surfactant type and concentrations, demonstrating satisfactory performance in terms of degreasing efficiency and composition of the washing wastewater. The degreasing efficiency of different enzyme-containing detergent formulations was studied on cotton fabric samples stained with triolein and compared to that of formulations containing only surfactant. The highest efficiency of the fatty soil removal in formulations with a low content of surfactants (0.4 %) was noted in the enzyme formulation containing Lutensol(A (R)) XP-80 (degreasing efficiency gt 80 %) and Triton(A (R)) X-100 (degreasing efficiency gt 60 %). An attempt was then made to optimize the composition of the enzyme formulation on the basis of one or both of these surfactants using statistically planned experiments and response surface methodology (RSM). Taking into consideration the environmental aspects and the shown detergency, it appeared that rather high degreasing effects were achieved in formulations based on a low quantities of Lutensol(A (R)) XP-80 (0.4 %) at all pH values. However, pH seemed to have a notable effect since the degreasing efficiency significantly increased with increasing pH and the amount of the enzyme. Formulations having a moderate alkaline pH profile and higher amount of enzyme exhibited a high cleaning performance of fatty soil even at a low concentration of the surfactant.", publisher = "Wiley, Hoboken", journal = "Journal of Surfactants and Detergents", title = "Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain", pages = "395-383", number = "3", volume = "18", doi = "10.1007/s11743-015-1674-x" }
Grbavčić, S., Marković, D., Rajilić-Stojanović, M., Antov, M., Sciban, M., Karadžić, I.,& Knežević-Jugović, Z.. (2015). Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain. in Journal of Surfactants and Detergents Wiley, Hoboken., 18(3), 383-395. https://doi.org/10.1007/s11743-015-1674-x
Grbavčić S, Marković D, Rajilić-Stojanović M, Antov M, Sciban M, Karadžić I, Knežević-Jugović Z. Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain. in Journal of Surfactants and Detergents. 2015;18(3):383-395. doi:10.1007/s11743-015-1674-x .
Grbavčić, Sanja, Marković, Darka, Rajilić-Stojanović, Mirjana, Antov, Mirjana, Sciban, Marina, Karadžić, Ivanka, Knežević-Jugović, Zorica, "Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain" in Journal of Surfactants and Detergents, 18, no. 3 (2015):383-395, https://doi.org/10.1007/s11743-015-1674-x . .